针刺研究2025，Vol.50Issue(8)：851-861,11.DOI:10.13702/j.1000-0607.20250181
电针通过中缝背核-基底外侧杏仁核环路缓解神经病理性疼痛痛觉敏化与焦虑样行为
Electroacupuncture alleviates neuropathic pain hyperalgesia and anxiety-like behaviors via the dorsal raphe nucleus-basolateral amygdala circuit
摘要
Abstract
Objective This study aims to investigate the intervention effects of electroacupuncture on neuropathic pain and anxiety-like behavior,and to explore the underlying mechanisms of the DRN5-HT-BLA(dorsal raphe nucleus serotoninergic-basolateral amygdala)neural circuit.Methods Male C57BL/6J mice were selected,and a neuropathic pain model was established through spared nerve injury(SNI)surgery.Electroacupuncture(100 Hz,0.3 mA,30 min)was applied to bilateral"Zusanli"(ST36)and"Sanyinjiao"(SP6).Anxiety-like behavior was assessed using the elevated plus maze(EPM)and open field(OF)tests,while mechanical allodynia was evaluated using the Von-Frey test.Viral tracing was used to confirm the anatomical connection between DRN5-HT and BLA.Immunofluorescence was used to assess the activity of 5-HT neurons in the DRN.Chemogenetic and calcium imaging techniques were employed to observe changes in the activity of 5-HT neurons in the DRN of DRN5-HT-BLA.Chemogenetics were used to observe the effect of inhibiting DRN5-HT-BLA on electroacupuncture.Results SNI model mice showed a significant decrease in mechanical pain threshold(P<0.000 1)and exhibited marked anxiety-like behavior in both the EPM and OF tests(P<0.01,P<0.05).Compared to the model group,electroacupuncture significantly increased the mechanical pain threshold in the electroacupuncture group(P<0.000 1).The EPM and OF results indicated that electroacupuncture significantly reduced anxiety-like behavior(P<0.05,P<0.001)without affecting spontaneous activity.Virus tracing results confirmed the anatomical connection between 5-HT neurons in the DRN and the BLA.Immunofluorescence results showed that the activity of 5-HT neurons in the DRN was significantly reduced(P<0.000 1)in SNI model mice,which was significantly reversed by electroacupuncture(P<0.01).Calcium imaging revealed that in SNI model mice,the excitability of 5-HT neurons in the DRN5-HT-BLA circuit was decreased,and electroacupuncture treatment restored their excitability.After chemogenetic inhibition of the DRN5-HT-BLA circuit,electroacupuncture failed to improve mechanical allodynia and anxiety-like behavior in the SNI model mice(P<0.05,P<0.001,P<0.01).Conclusion 5-HT neurons in the DRN5-HT-BLA circuit play a crucial role in the mechanical allodynia and associated anxiety-like behavior in neuropathic pain.Electroacupuncture activates this circuit,enhancing the activity of 5-HT neurons in the DRN,thereby alleviating mechanical allodynia and anxiety-like behavior in SNI model mice.关键词
神经病理性疼痛/焦虑/电针/中缝背核/杏仁核/5-羟色胺能神经元Key words
Neuropathic pain/Anxiety/Electroacupuncture/Dorsal raphe nucleus/Basolateral amygdala/Serotoninergic(5-HT)neurons引用本文复制引用
吴宗霖,吴媛媛,徐赟赟,陈月蓉,刘哲,童思媛,王林宝,陈亮,王展鹏,方剑乔..电针通过中缝背核-基底外侧杏仁核环路缓解神经病理性疼痛痛觉敏化与焦虑样行为[J].针刺研究,2025,50(8):851-861,11.基金项目
国家自然科学基金面上项目(No.82074541) （No.82074541）
