中国输血杂志2025，Vol.38Issue(8)：1030-1034,1049,6.DOI:10.13303/j.cjbt.issn.1004-549x.2025.08.005
中国人群弱部分D15型的精准检测及其对输血安全潜在影响的评估与应对策略
Precise detection of weak partial D type 15 in the Chinese population:evaluation of their potential impact on blood transfusion safety and development of appropriate response strategies
摘要
Abstract
Objective To investigate the precise detection methods for weak partial D type 15 and evaluate their impli-cations for blood transfusion safety,along with the development of corresponding strategies.Methods A combination of se-rological methods,including the microplate method,indirect antiglobulin tube method,and microcolumn gel card method,was employed to identify RhD-negative and RhD variant samples.RhD-negative samples were screened for the presence of RHD genes using whole-blood direct PCR amplification.Subsequently,RhD variant samples and RhD-negative samples con-taining RHD genes underwent full-coding-region sequencing of the RHD gene to confirm their genotypes.The genotyping re-sults were further correlated with the serological test findings for comprehensive analysis.Results Among 615 549 first-time healthy blood donors,3 401 samples with an RhD-negative phenotype and 156 samples with RhD variant were identi-fied.Of the 3 401 RhD-negative samples,1 054 were found to harbor RHD genes.Gene sequencing analysis of the 156 RhD variants and the 1 054 serological negative samples revealed that 89 samples contained the RHD*15(c.845G>A)al-lele.Conclusion The integration of serological testing methods and genotyping technologies for the precise determination of RhD blood type plays a critical role in ensuring the safety and compatibility of blood transfusions.关键词
弱部分D15型/RhD变异型/精准检测/输血安全Key words
weak partial D type 15/RhD variant/precise detection/blood transfusion safety分类
医药卫生引用本文复制引用
章旭,周助人,黄旭颖,李丽春,李伟伟,侯萍,李晓丰,李剑平..中国人群弱部分D15型的精准检测及其对输血安全潜在影响的评估与应对策略[J].中国输血杂志,2025,38(8):1030-1034,1049,6.基金项目
沈阳市血液安全研究重点实验室,沈阳市科技创新平台建设计划项目(21-104-0-15) （21-104-0-15）
沈阳市科技计划项目(24-214-3-118) （24-214-3-118）
