中国输血杂志2025，Vol.38Issue(8)：1050-1055,1062,7.DOI:10.13303/j.cjbt.issn.1004-549x.2025.08.008
MNS杂交糖蛋白GP(B-A-B)在广东与贵州少数民族中的分布
Distribution of GP(B-A-B)hybrid glycophorins in Guangdong&Guizhou minority populations
摘要
Abstract
Objective To investigate the distribution of GP(B-A-B)hybrid glycophorins in several Chinese minority populations from southern regions of China(Guangdong&Guizhou).Methods Whole blood samples were collected from 536 blood donors representing 15 different Chinese ethnic minority groups,including She,Bouyei,Yi and Miao,as well as Chuanqing populations.Genomic DNA was extracted and GYP(B-A-B)genotyping was conducted by high resolution melting(HRM)minority method using the GYPB pseudoexon 3-specific primers.Direct sequencing of GYPB pseudoexon 3 was per-formed in the samples with variant curves.Results Only one genotype of GP(B-A-B)hybrid glycophorins(GYP*Mur/GYPB)was identified among these 536 samples.In total,15 She(15/162,9.26%),18 Bouyei(18/113,15.93%),3 Yi(3/79,3.80%),3 Chuanqing(3/45,6.67%),2 Bai(2/42,4.76%),3 Miao(3/40,7.50%),1 Shui(1/12,8.33%),2 Gelao(2/12,16.67%),1 Tujia(1/8,12.50%)and 1 Dong(1/6,16.67%)blood donors with heterozygous GYP*Mur allele were identified.Among 8 Hui,5 Manchu,2 Mongolian,1 Yao and 1 Li donors,no GYP(B-A-B)hybrid gene carrier was found.In addition,four nucleotide polymorphisms(SNPs)were identified in 6 samples with a variant melt-ing curve detected by HRM.Conclusion GP.Mur is the most common type of GP(B-A-B)hybrid glycophorins among Chinese minority populations,with frequency varying across different populations.It is recommended to involve GP.Mur re-agent cells in the antibody screening cells for populations with a high frequency of GYP*Mur allele.关键词
MNS杂交糖蛋白/GP(B-A-B)/GP.Mur/高分辨率熔解曲线分析(HRM)Key words
MNS hybrid glycophorin/GP(B-A-B)/GP.Mur/high resolution melting(HRM)分类
医药卫生引用本文复制引用
魏玲,汪鹏,温机智,贾双双,姬艳丽..MNS杂交糖蛋白GP(B-A-B)在广东与贵州少数民族中的分布[J].中国输血杂志,2025,38(8):1050-1055,1062,7.基金项目
广州市科技计划项目(2024A03J0373) （2024A03J0373）
广州市医学重点学科(2025-2027) （2025-2027）
