中国输血杂志2025，Vol.38Issue(8)：1056-1062,7.DOI:10.13303/j.cjbt.issn.1004-549x.2025.08.009
固定献血者红细胞基因数据库的建立和应用
Establishment and application of a red blood cell gene database in regular blood donors
摘要
Abstract
Objective To establish a"regular blood donor red blood cell gene database"(hereafter referred to as the"database")by applying molecular biology techniques for red blood cell antigens genotyping and utilizing information technol-ogy software,and to determine the significance and application value of this"database"in precise red blood cell transfu-sion.Methods Fifteen antigens[C,c,E,e,M,N,S,s,Fy(a),Fy(b),Jk(a),Jk(b),Le(a),Le(b),P1]across six blood group systems(RHCE,MNS,FY,JK,Lewis and P1PK)were detected among 9 426 regular blood donors using the TaqMan-MGB method combined with an improved U-shaped microplate approach.With the assistance of informa-tion technology software,the"database"was integrated into the overall inventory management system of the blood supply chain.This enabled comprehensive management of regular blood donor and patient information,test results,specific antigen screening for regular blood donors,graded antigen matching between donors and patients,and rare blood type donor re-cords.Results The TaqMan-MGB method successfully detected paired antigens(C/c,E/e,M/N,S/s,Fya/Fyb,Jka/Jkb)within a single reaction well using a standardized PCR amplification protocol.This method provided a reliable testing solution for clinical institutions and empowered blood collection and supply organizations with high-throughput screening ca-pabilities.In the blood supply chain,genotyped red blood cells accounted for 13.2%(721/5 462 U)of the total inventory,with 95.34%(348/365)originating from donors who donated two units of blood.Moreover,the"database"fulfilled 94.06%(443/471 U)of compatible transfusion requirements from medical institutions and effectively managed rare blood type donors.Conclusion The establishment of the"database"facilitated the transition of blood compatibility testing from traditional serological methods to molecular biology-based gold standard techniques,significantly advancing the implementa-tion of precise transfusion strategies based on multi-antigen matching between donors and patients.关键词
红细胞血型抗原基因分型/数据库/精准输血/红细胞输注无效Key words
red blood cell antigen genotyping/database/precise blood transfusion/red blood cell transfusion refracto-riness分类
医药卫生引用本文复制引用
冯智慧,迟晓云,胡彬,刘丽,李大玮,逄淑涛..固定献血者红细胞基因数据库的建立和应用[J].中国输血杂志,2025,38(8):1056-1062,7.基金项目
青岛市医疗卫生重点学科建设项目 （）
山东省输血协会科研基金资助项目(No.2024-sdsx03) （No.2024-sdsx03）
