中国兽医杂志2025，Vol.61Issue(8)：53-61,9.DOI:10.20157/j.cnki.zgsyzz.2025.08.008
藏猪猪细小病毒(XZ-PPV株)生物信息学分析
Bioinformatic Analysis of Tibetan Porcine Parvovirus(XZ-PPV Strain)
摘要
Abstract
To elucidate the bioinformatic characteristics of the nonstructural protein 1(NS1)and viral structural protein 2(VP2)of Tibetan porcine parvovirus(PPV),this study conducted polymerase chain reaction(PCR)and full-genome sequencing on blood samples from suspected diseased Tibetan native pigs.Positive samples underwent virus isolation and cytopathic effect(CPE)observation.A phylogenetic tree of the isolated PPV strain was constructed to analyze genetic relatedness.Online tools and software were used to perform bioinformatic analysis of the amino acid sequences of NS1 and VP2,including their physicochemical properties and spatial structures.The results showed that one local PPV strain was successfully identified through PCR and whole-genome sequencing,with a genome length of 4 336 bp.Based on regional origin,it was named PPV/China/XZ(XZ-PPV).Phylogenetic analysis indicated that XZ-PPV is most closely related to Chinese reference strains KX233726 TJ and KJ201927,as well as German strains JN400516 7a and JN400517 8a.Bioinformatic analysis revealed that both NS1 and VP2 of XZ-PPV are hydrophilic,stable proteins with no signal peptides or transmembrane domains.Their secondary structures are primarily composed of random coils,and the overall spatial conformations are relatively stable.In conclusion,XZ-PPV shows differences in amino acid hydrophilicity and protein spatial structure compared to previously known PPV strains.For example,differences and similarities exist in the amino acid sites within the hydrophilic regions of the NS1 and VP2 proteins,as well as in the proportions of their secondary structures(such as α-helices and β-turns).These findings provide valuable data for the future diagnosis of PPV,vaccine development,and offer theoretical insights for prevention and treatment strategies.关键词
藏猪/猪细小病毒(PPV)/生物信息学分析/二级结构/NS1蛋白/VP2蛋白Key words
Tibetan pig/porcine parvovirus(PPV)/bioinformatic analysis/secondary structure/NS1 protein/VP2 protein分类
农业科技引用本文复制引用
叶霄霞,强巴央宗,罗侣,聂浩洋,刘福洲,李海洋,廉帅,纪菁蕊,韦明邦,商鹏..藏猪猪细小病毒(XZ-PPV株)生物信息学分析[J].中国兽医杂志,2025,61(8):53-61,9.基金项目
国家重点研发计划(2022YFD1600903) （2022YFD1600903）
国家自然科学基金地区科学基金项目(32160773) （32160773）
西藏自治区科技计划项目(XZ202202JD0002N) （XZ202202JD0002N）
西藏自治区重大科技专项项目(XZ202101ZD0005N) （XZ202101ZD0005N）
