中国畜牧杂志2025，Vol.61Issue(8)：77-84,8.DOI:10.19556/j.0258-7033.20250415-12
单细胞转录组测序技术及其在猪研究中的应用进展
Single-Cell Transcriptomic Sequencing Technologies and Their Application Progress in Pig Research
摘要
Abstract
Single-cell RNA sequencing(scRNA-seq)is a technology that enables the acquisition of whole-genome expression profiles at the individual cell level.This is achieved through a workflow involving cell isolation,library preparation,high-throughput sequencing,and subsequent bioinformatic analysis.scRNA-seq provides comprehensive gene expression data at single-cell resolution,which is instrumental for investigating gene expression heterogeneity within specific cell subpopulations.This capability facilitates the identification of previously unknown or rare cell types and establishes a critical foundation for in-depth mechanistic studies.This review summarizes the methodologies and explores their applications in swine research,with a focus on genetic breeding,disease resistance,and growth and development.Furthermore,the current limitations and future prospects of scRNA-seq technology in these fields are discussed.关键词
单细胞转录组测序/猪/遗传育种/抗病性/生长发育Key words
Single-cell RNA sequencing/Swine/Genetic breeding/Disease resistance/Growth and development分类
农业科技引用本文复制引用
牛欣然,郑卓也,邢凯,王楚端..单细胞转录组测序技术及其在猪研究中的应用进展[J].中国畜牧杂志,2025,61(8):77-84,8.基金项目
国家重点研发计划(2023YFD1300400) （2023YFD1300400）
农业生物育种国家科技重大专项(2023ZD0404604) （2023ZD0404604）
财政部和农业农村部:国家现代农业产业技术体系(CARS-35) （CARS-35）
