转化医学杂志2025，Vol.14Issue(7)：145-149,5.DOI:10.3639/j.issn.2095-3097.2025.04.028
黄芪润肠方治疗气阴两虚型便秘小鼠的疗效及相关机制
Effect and Mechanism of Huangqi Runchang Prescription in Alleviating Constipation of Mice with Deficiency of Qi and Yin
摘要
Abstract
Objective To explore the effect and mechanism of Huangqi Runchang prescription on constipation based on the mouse model of Qi-Yin deficiency.Methods Male C57BL/6J mice were divided into the control group,the consti-pation group,the Huangqi Runchang prescription low-,medium-and high-dose groups[0.091 5,0.183,0.366 g/(kg·d)]and positive control group[6.2 mL/(kg·d)lactulose],with 10 mice in each group.The constipation model of Qi and Yin deficiency was established by a combination of improper diet+hot drug administration+weight-bearing swimming.After corresponding drug intervention for 14 days,the defecation parameters(time to first black stool,fecal water content and total fecal matter)were compared among the groups.The following parameters were analyzed:gastrointestinal motility biochemical indexes[mo-tilin,gastrin,endothelin,Substance P,and vasoactive intestinal peptide];liver and kidney function indexes[aspartate amino-transferase,alanine aminotransferase,albumin and serum creatinine];small intestinal pathological changes;and expression of tight-junction related proteins.Results Compared with the constipation group,the time of first black stool excretion in the Huangqi Runchang prescription medium-and high-dose groups and the positive control group was significantly decreased,while total feces output and fecal water content were increased(P<0.05).Serum levels of motilin,gastrin,Substance P,and endothelin-1 increased(P<0.05),whereas vasoactive intestinal peptide levels decreased significantly(P<0.05).There were no significant differences in the serum levels of aspartate aminotransferase,alanine aminotransferase,albumin,or creatinine among all groups.The degree of structural damage in small intestinal tissue was significantly improved.The relative expression levels of occludin,claudin-1 and zonula occludens-1 in the small intestine increased.Conclusion Huangqi Runchang pre-scription can effectively relieve Qi-Yin deficiency-induced constipation symptoms in mice by regulating tight-junction-relat-ed protein expression,repairing the small intestinal mucosa,and improving gastrointestinal function.关键词
便秘/气阴两虚型/黄芪润肠方/小鼠,近交C57BL/疾病模型,动物/紧密连接蛋白质类Key words
Constipation/Qi-yin deficiency type/Huangqi Runchang Decoction/Mice,inbred C57BL/Disease models,animal/Tight junction proteins分类
医药卫生引用本文复制引用
王永通,季利江,蒋捷,丁旭枫,黄华..黄芪润肠方治疗气阴两虚型便秘小鼠的疗效及相关机制[J].转化医学杂志,2025,14(7):145-149,5.基金项目
常熟市科技发展计划项目(CS202450,CS202227) （CS202450,CS202227）
常熟市卫生健康委员会科技计划项目(CSWS202103) （CSWS202103）
