中医临床研究2025，Vol.17Issue(11)：18-26,9.DOI:10.3969/j.issn.1674-7860.2025.11.004
基于超高效液相色谱-四级杆-飞行时间串联质谱技术和网络药理学探讨华山参滴丸治疗慢性阻塞性肺疾病的药效物质基础及作用机制
A study on the pharmacological substance basis and action mechanism of Huashanshen Diwan in the treatment of COPD based on UPLC-Q-TOF-MS/MS technology and network pharmacology
摘要
Abstract
Objective:To explore the pharmacological substance and underlying action mechanism of Huashanshen Diwan(华 山参滴丸)in the treatment of chronic obstructive pulmonary disease(COPD).Methods:The components in plasma of Huashanshen Diwan were analyzed by UPLC-Q-TOF-MS/MS technology,and the active ingredients were screened out by the Swiss ADME platform further.The active ingredient corresponding targets were searched by the Swiss Target Prediction and SuperPred databases,and COPD disease targets were sorted out by the GeneCards database.The intersection targets of ingredient targets and disease targets were taken to obtain underlying targets of Huashanshen Diwan in the treatment of COPD.A protein-protein interaction network was constructed by STRING,and a medicine-active ingredient-intersection target interaction network was obtained by Cytoscape 3.9.1 software to further screen out core targets.The GO functional enrichment analysis and KEGG pathway enrichment analysis were performed to explore the possible action mechanism of underlying targets,and molecular docking validation was performed on core active components and core targets by CB-Dock.Results:A total of 19 components in plasma were identified from Huashanshen Diwan,and 288 underlying targets were screened out,of which 56 were core targets.The GO functional enrichment analysis and KEGG pathway enrichment analysis showed that calcium signaling pathway,PI3K/Akt signaling pathway,cAMP signaling pathway,MAPK signaling pathway,Ras signaling pathway were the key signaling pathways of Huashanshen Diwan in the treatment of COPD,and ESR1,ALB,EGFR,STAT3,and PTGS2 were the key targets.The results of molecular docking showed that formononetin,biochanin A,anisodamine,scoparone,palmic acid had good binding activity with the core targets,which were the main active substances of Huashanshen Diwan in the treatment of COPD.Conclusion:Huashanshen Diwan can treat COPD with multiple components,multiple targets,and multiple pathways,providing a basis for in-depth biological experiment.关键词
华山参滴丸/慢性阻塞性肺疾病/网络药理学/物质基础/机制Key words
Huashanshen Diwan/Chronic obstructive pulmonary disease/Network pharmacology/Material basis/Mechanism分类
医药卫生引用本文复制引用
栾哲宇,王晗笑,彭鑫,张益豪,李云辉,封继宏..基于超高效液相色谱-四级杆-飞行时间串联质谱技术和网络药理学探讨华山参滴丸治疗慢性阻塞性肺疾病的药效物质基础及作用机制[J].中医临床研究,2025,17(11):18-26,9.基金项目
青年岐黄学者培养项目(国中医药人教函[2022]256号) （国中医药人教函[2022]256号）
国家自然科学基金重点支持项目(U20A20398). （U20A20398）
