安徽农业科学2025，Vol.53Issue(16)：141-148,8.DOI:10.3969/j.issn.0517-6611.2025.16.032
不同根瘤菌和栽培基质对云南主栽蚕豆品种"云早15"和"陵西一寸"结瘤和固氮效果的影响
Effects of Different Rhizobia Strains and Culture Substrates on Nodulation and Nitrogen Fixation of Two Faba Bean Varieties"Yunzao No.15"and"Lingxiyicun"in Yunnan Province
摘要
Abstract
[Objective]The substitution of vegetables with faba beans,coupled with the screening and inoculation of highly efficient nitrogen-fixing and broadly adaptive rhizobia,holds promise for fostering the sustainable development of vegetable-tobacco continuous cropping.[Meth-od]Two pot experiments were conducted,employing two faba bean varieties cultivated in Yuxi City,which named"Yunzao No.15"(YZ-15)and"Lingxiyicun"(LX-1).High-efficiency and high-compatibility rhizobia from 10 rhizobia strains with diverse genetic backgrounds were screened.For designated efficient rhizobia strains,a comparison of the effects of three different culture substrates on nodulation and nitrogen fix-ation was carried out.The shoot dry weight,root dry weight,SPAD,nodule number and nodule dry weight were measured.[Result]Compared with the control,the nodule number,nodule dry weight,shoot dry weight,root dry weight and SPAD of faba bean were significantly increased af-ter inoculation with the above 10 rhizobia strains.The symbiosis between"Yunzao No.15"with YX-2-26,WS-1-13,YL-1-23 and YL-1-35 ex-hibited significantly better effects than with the other 6 rhizobia strains,while the symbiosis of"Lingxiyicun"with WS-1-13,YX-2-26 and WS-1-18 was more favorable.Co-inoculation of WS-1-13 and YX-2-26 demonstrated excellent nodulation and nitrogen fixation effects in soil sub-strate that better than those in vermiculite substrate,leading to a significant increase in nodule number,nodule dry weight,shoot dry weight,root dry weight and SPAD.[Conclusion]This study successfully screened two rhizobia strains with high efficiency,broad-spectrum compatibility and strong adaptability,providing high-quality germplasm resources for the development and application of faba bean rhizobia inoculant in Yunnan Province.关键词
根瘤菌/云早15/陵西一寸/适配性/栽培基质/适应性Key words
Rhizobia/Yunzao No.15/Lingxiyicun/Compatibility/Culture substrate/Adaptability分类
农业科技引用本文复制引用
蒲怡,王妍,朱佳豪,代快,李江舟,焦健,崔永和,何泉,林杉..不同根瘤菌和栽培基质对云南主栽蚕豆品种"云早15"和"陵西一寸"结瘤和固氮效果的影响[J].安徽农业科学,2025,53(16):141-148,8.基金项目
中国烟草总公司云南省公司重点科技计划项目(20215300-00241032,2024530000241020) （20215300-00241032,2024530000241020）
三亚崖州湾科技城管理局资助项目(SYND-2021-10). （SYND-2021-10）
