生物资源2025，Vol.47Issue(4)：335-340,6.DOI:10.14188/j.ajsh.20250102001
长江口河蚬染色体制备及核型分析
Chromosome preparation and karyotype analysis of Corbicula fluminea from the Yangtze River estuary
摘要
Abstract
Corbicula clams inhabit both saltwater and freshwater bodies,and their karyotypes and ploidy levels show regular distribution,making them a potential model for studying the evolution of bivalves from seawater to freshwater.In this study,Corbicula fluminea from the Yangtze River estuary was used as the material,and the samples were collected from Dongtan,Chongming Island,Shanghai.After the axe foot was injected with 5 mg/mL phytohemagglutinin(PHA)at a rate of 4 μg/g wet weight for 25 h and 10 mg/mL colchicine at a rate of 2 μg/g wet weight for 5 h sequentially,the gill tissue of C.fluminea was dissected and subjected to hypoosmotic treatment,fixation,chromosome preparation and staining for observation of the chromosome characteristics and karyotype analysis of C.fluminea from the Yangtze River estuary.The results showed that C.fluminea from the Yangtze River estuary had 2n=38 chromosomes,and the 19 pairs of chromosomes can be assigned into 2 pairs of metacentric chromosome(m),8 pairs of submetacentric chromo-some(sm),8 pairs of subtelocentric chromosome(st),and 1 pair of telocentric chromosome(t).The karyotype of C.fluminea from the Yangtze River estuary was 2n=38=4m+16sm+16st+2t,with no satellite or heterosomes ob-served.关键词
河蚬/染色体/核型/遗传分化Key words
Corbicula fluminea/chromosome/karyotype/genetic differentiation分类
生物科学引用本文复制引用
周思洁,唐杨欣,皮杰,张廖瑞林,王旭,刘新华,向建国,李德亮..长江口河蚬染色体制备及核型分析[J].生物资源,2025,47(4):335-340,6.基金项目
国家自然科学基金(31772832) （31772832）
国家重点研发计划(2023YFD2400900) （2023YFD2400900）
湖南省重点研发计划(2022NK2060) （2022NK2060）
