生物资源2025，Vol.47Issue(4)：379-388,10.DOI:10.14188/j.ajsh.20250721002
基于网络药理学探讨柯孜木克颗粒治疗慢性细菌性前列腺炎作用机制
Mechanism of Kezmekh granules in the treatment of chronic bacterial prostatitis based on network pharmacology
摘要
Abstract
Based on network pharmacology and animal experiments,this study investigated the preventive and thera-peutic effects of Kezmekh(KZMK)granules,a traditional Chinese medicine and ethnic medicine,on chronic bacterial prostatitis(CBP).Network pharmacology methods were used to predict the core targets and signaling pathways of KZMK granules acting on CBP,and a total of 181 potential targets were screened,primarily involving four key signaling pathways,with KEGG enrichment analysis confirming the phosphatidylinositol 3-kinase-protein kinase B(PI3K-AKT)sig-naling pathway as the most significantly associated with CBP.To validate these predictions,the study successfully estab-lished a rat CBP model induced by Escherichia coli.Hematoxylin and eosin(HE)staining revealed that KZMK signifi-cantly improved prostate tissue histopathology and reduced inflammatory cell infiltration.Enzyme-linked immunosorbent assay(ELISA)demonstrated that KZMK markedly decreased serum levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)(P<0.05 or P<0.01).Western blot analysis confirmed that KZMK significantly downregulated the expression of PI3K,AKT,as well as TNF-α,IL-1β,and IL-6 proteins in prostate tissues(P<0.001 or P<0.01).In summary,these findings suggest that KZMK may exert its therapeutic effects on CBP by inhibiting the re-lease of pro-inflammatory factors IL-6 and TNF-α,regulating the phosphorylation of key proteins in the PI3K-AKT sig-naling pathway,thereby alleviating inflammatory responses in prostate tissues.关键词
柯孜木克颗粒/慢性细菌性前列腺炎/网络药理学/PI3K-AKT信号通路Key words
Kezmekh granules/chronic bacterial prostatitis(CBP)/network pharmacology/PI3K/AKT signaling pathway分类
医药卫生引用本文复制引用
努尔巴合提·候瓦提,胡梦颖,叶力夏提·达那别克,程波,徐磊,哈木拉提·哈斯木..基于网络药理学探讨柯孜木克颗粒治疗慢性细菌性前列腺炎作用机制[J].生物资源,2025,47(4):379-388,10.基金项目
自治区公益性科研院所基本科研业务费项目(KY2021099) （KY2021099）
自治区卫生健康青年医学科技人才专项科研项目(WJWY-202224) （WJWY-202224）
