分析测试学报2025，Vol.44Issue(9)：1723-1726,4.DOI:10.12452/j.fxcsxb.25073101
纳米孔检测RNA单核苷酸突变和修饰新方法
A New Method for Detecting Single Nucleotide RNA Mutations and Modifications Using Nanopores—RNA-SCAN
摘要
Abstract
RNA plays a crucial role in biological processes,and its single-nucleotide mutations and modifications are closely associated with various diseases.However,existing RNA detection meth-ods are limited in widespread application due to their operational complexity and analytical challeng-es.Consequently,the development of analytical techniques capable of precisely identifying RNA sin-gle-nucleotide mutations and modifications is essential.Among various approaches,nanopore single-molecule technology demonstrates significant potential.Recently,Nature Nanotechnology reported a novel method utilizing solid-state glass nanopores for detecting single-nucleotide RNA mutations and modifications—RNA single-nucleotide characterization and analysis nanolatch(RNA-SCAN).By in-tegrating RNA/DNA origami nanotechnology with programmable nanolatches,RNA-SCAN achieves precise identification of single-nucleotide variations within complex RNA structures without requiring labeling or amplification steps.This technology presents a novel approach for detecting RNA-related diseases and provides a powerful tool for in-depth research into RNA properties,further expanding the applications of nanopore technology.关键词
RNA/单核苷酸突变和修饰/纳米孔/纳米锁Key words
RNA/single-nucleotide mutations and modifications/nanopore/nanolatch分类
化学化工引用本文复制引用
郝文英,吴海臣..纳米孔检测RNA单核苷酸突变和修饰新方法[J].分析测试学报,2025,44(9):1723-1726,4.基金项目
国家自然科学基金资助项目(22025407) （22025407）
