分析测试学报2025，Vol.44Issue(9)：1727-1740,14.DOI:10.12452/j.fxcsxb.25062303
基于框架核酸的高灵敏多元生物分析
Highly Sensitive Multiplexed Biomarker Detection Based on Framework Nucleic Acids
摘要
Abstract
With the advancement of precision medicine,there is a growing demand for high-through-put platforms capable of detecting multiple biomarkers.Framework nucleic acids(FNAs),owing to their highly programmable structures and functional scalability,have emerged as ideal materials for the construction of next-generation bioanalytical platforms.FNAs enable highly sensitive detection of various biomarkers,including microRNA(miRNAs),proteins,and metabolites,with exceptional specificity and signal amplification capabilities,making them particularly suitable for multiplexed and synergistic target recognition.The integration of FNAs with microfluidic chips and portable detec-tion devices further enhances detection throughput and sensitivity,facilitating their translational ap-plications in clinical scenarios such as early disease screening,personalized intervention,and point-of-care diagnostics.This review systematically summarizes recent progress and representative applica-tions of FNAs in multiplexed bioanalysis,focusing on the design of recognition elements,sensing in-terfaces,spatial configuration control,signal amplification strategies,hardware integration,and signal acquisition.The aim is to provide theoretical support and practical guidance for the develop-ment of robust multi-biomarker detection systems.关键词
框架核酸(FNAs)/多标志物检测/微流控芯片/便携式检测Key words
framework nucleic acids/multiplexed biomarker detection/microfluidic chip/porta-ble detection device分类
化学化工引用本文复制引用
闫庆龙,夏凯,冯世伦,彭红珍,王丽华..基于框架核酸的高灵敏多元生物分析[J].分析测试学报,2025,44(9):1727-1740,14.基金项目
国家重点研发计划项目(2023YFB3208204) （2023YFB3208204）
国家自然科学基金青年科学基金项目(12305400) （12305400）
祥符实验室开放项目(XF012022E0100) （XF012022E0100）
