黑龙江动物繁殖2025，Vol.33Issue(4)：17-22,6.DOI:10.19848/j.cnki.ISSN1005-2739.2025.08.0003
体外生产绵羊早期胚胎发育异常分析
Analysis of developmental abnormalities in early sheep embryos produced in vitro
摘要
Abstract
Early developmental abnormalities of embryos in domestic animals represents a considerable economic loss.The experiment aimed to investigate the early developmental abnormalities of in vitro produced sheep embryos.Immature oocytes from sheep and mice were collected and used for in vitro maturation and fertilization,respectively.During the in vitro embryo culture,the developmental processes were tracked for counting the normal developmental rates and the proportions of embryos at different stages of development at different time points.Subsequently,immunofluorescence staining was used to detect the cell proliferation and differentiation of sheep blastocysts.The results showed that the blastocyst formation and hatching rates of in vitro produced sheep embryos were significantly lower compared to those of either in vitro produced mouse embryos or sheep embryos(P<0.05).About 11.27%and 53.90%of in vitro produced sheep embryos were arrested at the 2～4 cell stage and 8～16 cell stage,respectively.The developmental delay was observed in the blastulation of some sheep embryos.Moreover,despite more trophectoderm cells in sheep blastocysts,the proportion and proliferation activity of trophectoderm cells were lower than in mouse blastocysts.In conclusion,the in vitro produced sheep embryos showed a high incidence of developmental abnormalities,primarily characterized by the arrest at the 8～16 cell stage and the delayed blastocyst development.关键词
绵羊/体外胚胎生产/发育不同步/滋养层/细胞增殖Key words
sheep/in vitro embryo production/developmental asynchrony/trophectoderm/cell proliferation分类
农业科技引用本文复制引用
李丽俐,程潇,徐尚荣,贾功雪..体外生产绵羊早期胚胎发育异常分析[J].黑龙江动物繁殖,2025,33(4):17-22,6.基金项目
青海省自然科学基金面上项目(2025-ZJ-998M) （2025-ZJ-998M）
中国科学院青年创新促进会人才项目(2021432) （2021432）
青海省"昆仑英才"·高端创新创业人才项目 （）
