黑龙江动物繁殖2025，Vol.33Issue(4)：40-45,6.DOI:10.19848/j.cnki.ISSN1005-2739.2025.08.0007
不同采卵方法对湖羊卵母细胞回收效果的影响
Effect of different ovum pick-up methods on oocyte recovery efficiency in Hu sheep
摘要
Abstract
This study aimed to compare the efficiency of conventional surgical ovum pick-up(OPU)versus laparoscopic ovum pick-up(LOPU),and to analyze the effects of operator skill level and different follicle-stimulating hormone(FSH)stimulation protocols on oocyte recovery outcomes in Hu sheep,aiming to optimize the oocyte collection technology system.A total of 180 adult Hu ewes were randomly allocated to three steps:①Comparison of recovery efficiency between surgical OPU and LOPU methods;②Analysis of the operator′s influence on LOPU outcomes;③Investigation of the effects of different FSH protocols:constant FSH dose,decreasing FSH dose and long-acting FSH.The results indicated that there was no significant difference in oocyte recovery rate between surgical and LOPU groups(P>0.05).However,the number of viable oocytes recovered per session in surgical group was significantly higher than that in LOPU group(P<0.05).Operator 2 achieved significantly higher oocyte recovery rates,average number of oocytes per session and number of viable oocytes compared with other operators(P<0.05).The decreasing FSH dose protocol yielded significantly higher average numbers of oocytes per session and viable oocytes than both the constant FSH dose and long-acting FSH groups(P<0.05).In conclusion,while LOPU offers the advantage of being minimally invasive,improving oocyte recovery efficiency requires enhancing operator technical skills and optimizing FSH protocols;conventional surgical OPU recovers more viable oocytes per session but causes greater trauma to the donor ewes.关键词
绵羊/湖羊/卵母细胞/腹腔镜活体采卵/促卵泡激素/手术采卵Key words
sheep/Hu sheep/oocyte/laparoscopic ovum pick-up(LOPU)/follicle-stimulating hormone(FSH)/surgical ovum pick-up(OPU)分类
农业科技引用本文复制引用
石笑笑,藤能斯·阿合麦提拜,艾拜地·买合苏木,吴向利,陈莹,艾比拜木·哈帕尔,林嘉鹏,汪立芹..不同采卵方法对湖羊卵母细胞回收效果的影响[J].黑龙江动物繁殖,2025,33(4):40-45,6.基金项目
科技创新2030-农业生物育种重大项目(2022ZD04013) （2022ZD04013）
新疆维吾尔自治区重大科技专项(2023A02011-2) （2023A02011-2）
新疆维吾尔自治区三农骨干人才培养项目(2023SNGGNT031) （2023SNGGNT031）
新疆维吾尔自治区现代农业羊产业技术体系项目(XJARS-09-08) （XJARS-09-08）
新疆维吾尔自治区科技援疆项目(2022E02017) （2022E02017）
