基础医学与临床2025，Vol.45Issue(9)：1165-1172,8.DOI:10.16352/j.issn.1001-6325.2025.09.1165
ANGPT1影响小鼠前列腺癌细胞系RM-1增殖、侵袭及血管生成
Effects of ANGPT1 on proliferation,invasion and angiogenesis of mouse prostate cancer cell line RM-1
摘要
Abstract
Objective To explore the regulatory mechanism of angiopoietin 1(ANGPT1)on proliferation,invasion and angiogenesis of prostate cancer cells.Methods Mouse prostate cancer cell line(RM-1)was divided into control group,NC-oe group,ANGPT1-oe group,NC-sh group and ANGPT1-sh group.NC-oe,ANGPT1-oe,NC-sh and ANGPT1-sh were transfected into RM-1 cells by Lipofectamine3000 reagent.The transfection efficiency was verified by RT-qPCR and Western blot,cell proliferation was detected by MTT assay and EdU stai-ning and cell invasion was detected by Transwell assay.The cells were divided into the following groups:control group,Tie2-expressing monocytes/macrophages(TEMs)group,NC-oe+TEM group,ANGPT1-oe+TEM group,NC-sh+TEM group and ANGPT1-sh+TEM groups.RM-1 and TEM were co-cultured.RM-1 cells were collected after 72 hours and subjected to MTT proliferation assay,EdU staining assay and Transwell invasion assay.The co-cultured cell supernatant from each group mouse umbilical vein endothelial cells(MUVECs)were co-incubated with cell supernatant collected from each group cells and then to detect the number of tubules formed.The co-cul-tured cell supernatant of each group was collected,and the level of MMP-9,VEGFA and COX-2 were detected by ELISA.Results 1)Compared with control group and NC-oe group,the level of ANGPT1 mRNA and protein in ANGPT1-oe group was increased(P＜0.05).Compared with control group and NC-sh group,the level of ANGPT1 mRNA and protein in ANGPT1-sh group was decreased(P＜0.05).2)Compared with control group,the cell viability,EdU positive rate,counting of invasive cells and of tubules formed in TEM group significantly increased(P＜0.05),the level of MMP-9,VEGFA and COX-2 in the supernatant was increased(P＜0.05).Compared with TEM group and NC-oe+TEM group,the cell viability,EdU positive rate,counting of invasive cells and of tubules formed in ANGPT1-oe+TEM group decreased(P＜0.05).The level of MMP-9,VEGFA and COX-2 in the supernatant was significantly decreased(P＜0.05).Compared with TEM group and NC-sh+TEM group,the cell viability,EdU positive rate,counting of invasive cell and of tubules formed in ANGPT1-sh+TEM group all increased(P＜0.05).The level of MMP-9,VEGFA and COX-2 in the supernatant were increased(P＜0.05).Conclusions The decreased expression of ANGPT1 in prostate cancer significantly enhances the promotion effect of TEMs on the proliferation,invasion and angiogenesis of prostate cancer cells,thus promoting the progression of prostate cancer.关键词
前列腺癌/血管生成素1/增殖/侵袭/血管生成Key words
prostate cancer/angiopoietin 1/proliferation/invasion/angiogenesis分类
医药卫生引用本文复制引用
安信,贾麟,马吾苏木·马木特,黄志鹏,卡哈尔曼·斯拉木,热夏提·热合曼,海日拉·吾拉木,库尔班江·阿布力克木,加素尔·巴吐尔..ANGPT1影响小鼠前列腺癌细胞系RM-1增殖、侵袭及血管生成[J].基础医学与临床,2025,45(9):1165-1172,8.基金项目
省部共建中亚高发病成因与防治国家重点实验室开放课题(SKL-HIDCA-2024-KY4) （SKL-HIDCA-2024-KY4）
"天山英才"医药卫生高层次人才培养项目(TSYC202301B078) （TSYC202301B078）
新疆维吾尔自治区自然科学基金(2021D01C017) （2021D01C017）
