口腔颌面外科杂志2025，Vol.35Issue(4)：260-267,8.DOI:10.12439/kqhm.1005-4979.2025.04.003
下颌升支截骨术后乳酸脱氢酶A对牙槽骨改建中破骨细胞作用的研究
Effect of lactate dehydrogenase A on osteoclasts during alveolar bone remodeling after mandibular ramus osteotomy
摘要
Abstract
Objective:To investigate the effect of lactate dehydrogenase A(LDHA)on alveolar bone remodeling after mandibular ramus osteotomy.Methods:The rat model of unilateral mandibular ramus osteotomy was established in vivo,and the rats were divided into the surgical group and the sham surgical group.Mouse RAW264.7 cells were induced to differentiate into osteoclasts and divided into normoxia group,hypoxia group,hypoxia+knockout(KO)group,hypoxia+siNC group,hypoxia+siLDHA group.The number of osteoclasts and LDHA expression levels in the local alveolar bone were detected using tartrate resistant acid phosphatase(TRAP)staining and immunofluorescence staining.The effects of hypoxia-inducible factor 1α(HIF-1α)on LDHA expression in osteoclasts under hypoxic conditions were assessed using real-time quantitative polymerase chain reaction(RT-qPCR),Western blotting,and TRAP staining.The impact of LDHA expression levels on the expression of osteoclast-specific genes was evaluated using immunofluorescence staining.Results:The osteoclasts in alveolar bone were active after mandibular ramus osteotomy,and the level of LDHA in alveolar osteoclasts increased after operation.Under hypoxic conditions in vitro,LDHA expression in osteoclasts was significantly increased compared to the normoxia group,and osteoclast differentiation capacity was also enhanced.Compared to the hypoxia group,the expression of osteoclast-specific genes was suppressed in the hypoxia+siLDHA group.Conclusion:LDHA after mandibular ramus osteotomy can accelerate alveolar bone remodeling by promoting osteoclast formation.关键词
下颌升支截骨术/低氧/乳酸脱氢酶A/破骨细胞/牙槽骨改建Key words
mandibular ramus osteotomy/hypoxia/lactate dehydrogenase A/osteoclast/alveolar bone remodeling分类
医药卫生引用本文复制引用
郑宇杉,唐燚,康非吾..下颌升支截骨术后乳酸脱氢酶A对牙槽骨改建中破骨细胞作用的研究[J].口腔颌面外科杂志,2025,35(4):260-267,8.基金项目
国家自然科学基金(82271013、82100955) （82271013、82100955）
中国博士后科学基金项目(2021M692429) （2021M692429）
