口腔颌面外科杂志2025，Vol.35Issue(4)：268-277,10.DOI:10.12439/kqhm.1005-4979.2025.04.004
LincRNA-EPS对牙龈卟啉单胞菌LPS诱导下小胶质细胞炎症反应的影响研究
Effect of lincRNA-EPS on the microglial inflammatory response induced by P.g-LPS:An experimental study
摘要
Abstract
Objective:To investigate whether long intergenic non-coding RNA-EPS(lincRNA-EPS)affects microglial inflammatory response induced by Porphyromonas gingivalis lipopolysaccharides(P.g-LPS).Methods:Mouse microglia cell lines(BV-2 cells)were stimulated with P.g-LPS in vitro and the expression changes of lincRNA-EPS were detected by real-time quantitative polymerase chain reaction(RT-qPCR).BV-2 cells were transfected with plasmids to construct lincRNA-EPS overexpressed BV-2 cells.The effects of lincRNA-EPS overexpression on P.g-LPS-induced migration ability of BV-2 cells were observed by cell scratch assay,and the expression changes of inflammation-related genes interleukin 1β(IL-1β),IL-6,tumor necrosis factor-α(TNF-α),inducible nitric oxide synthase(iNOS),arginase-1(Arg1),nuclear factor kappa B protein family member p65(NF-κB p65)and phosphorylated p65(pp65)were detected by RT-qPCR,enzyme-linked immunosorbent assay(ELISA)and Western blotting.The subcellular localization of p65 in BV-2 cells was detected by immunofluorescence staining.Results:The expression of lincRNA-EPS was down-regulated after in vitro P.g-LPS induction of BV-2 cells(P<0.05).Overexpression of lincRNA-EPS significantly inhibited the P.g-LPS-induced migration of BV-2 cells and the expressions of IL-1β,IL-6,TNF-α,and iNOS(P<0.05).In contrast,the expression of Arg1 was up-regulated at resting state(P<0.05).After overexpression of lincRNA-EPS,the expression of total p65 in BV-2 cells was not significantly changed,while the p65 in the nucleus and pp65 expression were decreased(P<0.05).Conclusion:This study demonstrated that lincRNA-EPS can attenuate the inflammatory response of BV-2 cells stimulated by P.g-LPS in vitro by inhibiting the phosphorylation and nuclear translocation of p65.关键词
长链非编码RNA-EPS/BV-2细胞/牙龈卟啉单胞菌脂多糖/炎症反应Key words
long intergenic non-coding RNA-EPS/BV-2 cells/Porphyromonas gingivalis lipopolysaccharide/inflammatory response分类
医药卫生引用本文复制引用
高建芳,苏俭生..LincRNA-EPS对牙龈卟啉单胞菌LPS诱导下小胶质细胞炎症反应的影响研究[J].口腔颌面外科杂志,2025,35(4):268-277,10.基金项目
国家自然科学基金(81873715、82170913) （81873715、82170913）
上海市科学技术委员会项目(201409006200) （201409006200）
