解放军医学院学报2025，Vol.46Issue(5)：468-475,8.DOI:10.12435/j.issn.2095-5227.24080803
TRPV4调控人诱导多能干细胞来源间充质干细胞成骨分化的体外细胞实验
TRPV4 regulates osteogenic differentiation of induced pluripotent stem cell derived mesenchymal stem cells in vitro
摘要
Abstract
Background Mesenchymal stem cells(MSCs)play a crucial role in bone defect repair and are widely used in clinical research.Studies have found that the transient receptor potential vanilloid channel protein 4(TRPV4)can promote osteogenesis in bone cells and is functionally expressed in osteoblasts,osteoclasts,and chondrocytes.There are multiple natural mutation sites on the human TRPV4,and mutations at these sites are associated with human skeletal pathologies such as brachydactyly,spondylometaphyseal dysplasia,and atrophic dysplasia.However,its role in MSCs remains unclear.Objective To investigate the effect of TRPV4 on osteogenic differentiation of mesenchymal stem cells(iMSCs)derived from human induced pluripotent stem cells(iPSCs).Methods iPSCs were induced into iMSCs and their phenotypes were detected by flow cytometry.The differentiation ability of osteoblastic,lipogenic and chondrogenic cells was evaluated.The expression of osteogenic genes and TRPV4 were found at various stages following osteogenic induction of iMSCs by using real-time quantitative PCR and Western blot,respectively.Alizarin red staining was used to detect the effects of TRPV4 small molecule agonist(GSK101)and inhibitor(GSK219)treatment of iMSCs on calcium salt deposition.iMSCs were cultured on different hardness substrates,and TRPV4 gene and protein expression were detected by real-time quantitative PCR and Western blot,respectively.Results The iMSCs induced by this experiment had the typical characteristics of mesenchymal stem cells.After osteogenic differentiation of iMSCs,the expressions of osteogenic mRNAs and the expressions of TRPV4 mRNA and protein all increased.The alizarin red staining area of iMSCs treated with TRPV4 agonist GSK101 increased significantly compared with the control group,which was opposite in the TRPV4 antagonist GSK219 group.mRNA and protein expression of TRPV4 gene increased after iMSCs were cultured on a cell culture plate with a high hardness substrate.Conclusion TRPV4 plays a positive regulatory role in the osteogenic differentiation of iMSCs.The increased hardness of the culture matrix can regulate the osteogenic differentiation of iMSCs by promoting TRPV4 expression.The results of this study expand the understanding of the osteogenic differentiation process of iMSCs,and provide new ideas for regulating the osteogenic differentiation of iMSCs and promoting the effect of clinical transplantation to repair bone defects.关键词
间充质干细胞/细胞分化/机械转导/成骨/离子通道Key words
mesenchymal stem cells/cell differentiation/mechanotransduction cellular/osteogenesis/ion channels分类
医药卫生引用本文复制引用
方文灿,田萧羽,朱彪,赵立升,杨烁,温宁..TRPV4调控人诱导多能干细胞来源间充质干细胞成骨分化的体外细胞实验[J].解放军医学院学报,2025,46(5):468-475,8.基金项目
国家自然科学基金项目(52072023) （52072023）
