农业环境科学学报2025，Vol.44Issue(7)：1893-1901,9.DOI:10.11654/jaes.2024-0819
秸秆源人工腐植酸的结构特征及其对玉米种子萌发的影响
Structural characteristics of straw-derived artificial humic acid and their effects on maize seed germination
摘要
Abstract
In order to investigate whether straw-derived artificial humic acids possess biological activity that stimulates plant growth,and to analyze the underlying mechanism based on the structural features of humic acids.In this study,artificial humic acid(A-HA)was synthesized from rice straw through a hydrothermal reaction,while weathered coal humic acid was selected as a representative of M-HA,which was used as a control.Elemental compositions,functional group types,and molecular weights of the two humic acids were analyzed.The effects of different concentrations of M-HA and A-HA solutions on seed germination and seedling growth of maize were studied,along with its potential mechanism.The results indicated that,in comparison with M-HA,A-HA exhibited a lower degree of humification and fewer acidic functional group,with a significant reduction in total acidity,carboxyl,and phenolic hydroxyl contents by 50.8%,41.7%,and 76.5%(P<0.05),respectively.However,A-HA contained a higher proportion of small molecule fractions compared to M-HA.Similar to M-HA,A-HA exhibited amphiphilic properties.However,the hydrophobic C in A-HA was primarily derived from alkyl-C(0-45 ppm)and aromatic-C(110-160 ppm),whereas M-HA's hydrophobic C was primarily derived from aromatic-C.Soaking maize seeds in solutions containing 1 mg·L-1(calculated as C,the same below)of M-HA and 10 mg·L-1 of A-HA resulted in a germination rate(GR)of 100%,and compared to the control treatment without humic acids,the germination index(GI),germination potential(GP),number of lateral roots(NLR),and length of lateral roots(LRL)were significantly improved by 51.7%and 29.5%,42.2%and 34.3%,48.1%and 33.3%,and 58.2%and 39.0%,respectively(P<0.05).In summary,although A-HA differs from M-HA in terms of structure and composition,it similarly stimulates seed germination and seedling growth,demonstrating its potential to be developed as a plant growth stimulant.关键词
水热反应/水稻秸秆/人工腐植酸/结构/种子萌发Key words
hydrothermal reaction/rice straw/artificial humic acid/structure/seed germination分类
农业科技引用本文复制引用
刘增,朱飘,潘文涛,石磊,骆艳娜,景建元,陈晓辉,熊启中,叶新新..秸秆源人工腐植酸的结构特征及其对玉米种子萌发的影响[J].农业环境科学学报,2025,44(7):1893-1901,9.基金项目
国家重点研发计划项目(2023YFD1700205) （2023YFD1700205）
国家自然科学基金项目(32402678) （32402678）
安徽省教育厅科研项目(2024AH050454) （2024AH050454）
安徽省大学生创新创业训练计划项目(S202310364240) National Key Research and Development Program of China(2023YFD1700205) （S202310364240）
National Natural Science Foundation of China(32402678) （32402678）
Anhui Provincial Department of Education Research Project(2024AH050454) （2024AH050454）
Anhui Provincial College Student Innovation and Entrepreneurship Training Program Project(S202310364240) （S202310364240）
