农业环境科学学报2025，Vol.44Issue(7)：1910-1919,10.DOI:10.11654/jaes.2024-0790
耐高温秸秆降解菌筛选鉴定及降解特性研究
Screening,identification and degradation characterization of high-temperature-tolerant straw-degrading bacteria
摘要
Abstract
The objective of this study was to identify high-temperature lignocellulosic-degrading bacteria from a paddy soil,establish a consortium of bacteria with high efficiency in lignocellulosic degradation,and investigate its degradation effect on straw,in order to promote the comprehensive utilization of agricultural waste.We obtained 11 degrading strains through preliminary screening by incubating for enrichment culture and using Congo red staining and aniline blue fading reaction.The enzymatic activities of cellulose,hemicellulose and lignin were measured.The highest β-D glucosidase activity was found in K6(716 U·mL-1),the highest cellulase activity was obtained in K1(393 U·mL-1),and the highest hemicellulase activity was found in K4(439 U·mL-1).The highest lignin peroxidase activity was found in strain R3(6.213 U·mL-1),the highest manganese peroxidase activity found in R1(3.989 U·mL-1),while the highest laccase activity was found in strain K5(2.593 U·mL-1).Based on the results of the transparent circle test,enzymatic activities of cellulose,hemicellulose and lignin,six strains(R2,R3,R4,K2,K5 and K6)were selected for molecular identification.The results showed that R2 was closely related to Priestia megaterium,R3 was closely related to Bacillus subtilis,R4 was closely related to Bacillus licheniformis,K2 was closely related to Priestia aryabhattai,K5 and K6 were closely related to Bacillus velezensis.Three consortia were constructed by using single strains.We found that consortium C(R4 and K6)had the best degrading effect,with the filter paper strip beginning to disintegrate on day 2 and turning into paper floss by day 5.After 15 days,the straw degradation rate was 23.69%higher than that of the control.The results suggest that the degradation effect of consortia was better than that of single bacterial colony.关键词
耐高温/秸秆降解菌/菌种鉴定/降解特性/复合菌群Key words
thermotolerant/straw degrading bacteria/identification/degradation characteristic/composite bacterial分类
资源环境引用本文复制引用
殷丽雯,莫迎平,耿天治,张娜,刘翠英,樊建凌..耐高温秸秆降解菌筛选鉴定及降解特性研究[J].农业环境科学学报,2025,44(7):1910-1919,10.基金项目
江苏省碳达峰碳中和科技创新专项资金项目(BE2022302) （BE2022302）
江苏省研究生实践创新计划项目(SJCX24_0487) （SJCX24_0487）
国家重点研发计划项目(2020YFA0607500) Special Funds for Carbon Peak and Carbon Neutral Science and Technology Innovation of Jiangsu Province(BE2022302) （2020YFA0607500）
Postgraduate Practice Innovation Program of Jiangsu Province(SJCX24_0487) （SJCX24_0487）
National Key Research and Development Program of China(2020YFA0607500) （2020YFA0607500）
