农业环境科学学报2025，Vol.44Issue(7)：1943-1954,12.DOI:10.11654/jaes.2024-0780
尿素配施硝化抑制剂DMPP对菜地土壤氮转化及N2O排放的影响
Effects of urea combined with nitrification inhibitor(DMPP)on soil nitrogen transformation and N2O emission in vegetable field
摘要
Abstract
Determining reasonable the amount of nitrification inhibitor added in high fertility vegetable soil is of great significance for improving nitrogen fertilizer utilization efficiency,reducing nitrogen loss,and greenhouse gas emissions.Taking the long-term vegetable planting soil around Erhai Lake as the object,3,4-dimethylpyrazole phosphate(DMPP)with conventional nitrogen application rate(300 kg·hm-2)of 0.5％,1％,3％,5％and 10％was added to study the changes of different forms of nitrogen concentration and N2O emission process during the cultivation process through indoor culture experiment.The results showed that compared with the control treatment(CK),the addition of DMPP prolonged the retention time of ammonium nitrogen(NH4+-N)in vegetable soil to 10-15 days,reducing the content of nitrate nitrogen(NO3--N)by 10.87％-16.21％and nitrite nitrogen(NO2--N)by 42.13％-52.99％.There was no significant difference between DMPP treatments with different dosages.The addition of DMPP reduced the net nitrogen mineralization rate,net nitrogen nitrification rate,and net ammonification rate of vegetable soil,but had no significant effect on the net assimilation rate.The addition of DMPP reduced the N2O emission flux and cumulative N2O emissions in vegetable soil,by 40.77％-45.32％and 49.06％-56.96％,respectively.However,there was no significant difference in soil N2O emissions among treatments with different amounts of DMPP.The addition of DMPP significantly reduced the abundance of soil AOB and Comammox genes,decreasing by 48.43％-67.90％and 21.82％-47.27％,respectively.As the concentration of DMPP increased,its inhibitory effect showed a trend of first weakening and then strengthening.The addition of DMPP promoted the abundance of soil AOA genes,with an increase of 55.61％-163.16％.The promotion effect became more significant with the increase of DMPP concentration.Therefore,the addition of DMPP effectively inhibited the conversion of NH4+-N to NO3--N and NO2--N,reduced the rate of soil nitrogen conversion,but had no significant effect on the net assimilation rate of soil nitrogen.At the same time,the addition of DMPP significantly reduced the abundance of soil AOB genes and promoted a decrease in N2O emissions,which is an effective measure for N2O emission reduction.Based on the inhibitory effect during cultivation,adding DMPP with a nitrogen application rate of 1％to high fertility vegetable soil is an economically feasible measure.关键词
硝化抑制剂/土壤氮转化速率/N2O排放/氨氧化微生物Key words
nitrification inhibitor/soil nitrogen transformation rate/N2O emission/ammonia oxidizing microorganism分类
资源环境引用本文复制引用
岩双,闫辉,王炽,蒋存征,李文明,陈安强,张丹..尿素配施硝化抑制剂DMPP对菜地土壤氮转化及N2O排放的影响[J].农业环境科学学报,2025,44(7):1943-1954,12.基金项目
云南省兴滇英才项目(202305AS350013) Xingdian Talent Project of Yunnan Province(202305AS350013) （202305AS350013）
