南京中医药大学学报2025，Vol.41Issue(8)：1047-1054,8.DOI:10.14148/j.issn.1672-0482.2025.1047
金丝桃苷对高糖诱导视网膜内皮细胞过度增殖的抑制作用
Inhibitory Effect of Hyperoside on Excessive Proliferation of Retinal Endothelial Cells Induced by High Glucose
摘要
Abstract
OBJECTIVE To investigate the effect of hyperoside on high glucose-induced excessive proliferation of retinal endo-thelial cells(RECs)and its possible mechanism.METHODS Diabetic retinopathy(DR)models were established in male Sprague-Dawley(SD)rats.DR rats were treated with low-and high-dose hyperoside(DR+L-HY group and DR+H-HY group).Additional-ly,the normal control(NC)group,DR non-intervention(DR)group and DR+calcium dobesilate intervention(DR+CD)group were set up.The differences in the number of RECs in retinal blood vessels were observed and compared among all groups after intervention.In addition,RECs were inoculated into cell culture plates after normal culture and subculture.They were divided into 5 groups according to different treatments:normal glucose(NG)group,high glucose(HG)group,mannitol(MT)group,high glucose+low concentration of hyperoside(HG+H100)group and high glucose+high concentration of hyperoside(HG+H400)group.The activ-ity,cell migration and tubule formation of RECs in each group were detected and compared by CCK-8,cell migration and tubule for-mation assays.Western blot and qPCR were used to detect the expression of NADPH Oxidase 4(NOX4)and thioredoxin interacting protein(TXNIP)in each group.RESULTS The number of RECs in the DR group was significantly increased compared to the NC group(P<0.01).In contrast,the DR+L-HY,DR+H-HY,and DR+CD groups all showed significant decreases in RECs number compared to the DR group(P<0.05,P<0.01),and the reduction of RECs in the DR+H-HY group was significantly greater than that in the DR+L-HY group(P<0.05).Furthermore,the cell activity,migration number and tube formation number of RECs in the HG group were significantly higher than those in the NG group(P<0.05,P<0.01).The protein and mRNA expression levels of NOX4 and TXNIP in the HG group were also significantly higher than those in the NG group(P<0.01).However,the RECs activity,RECs mi-gration number and tube formation number in the HG+H100 group and the HG+H400 group were significantly lower than those in the HG group(P<0.05,P<0.01).The expression levels of NOX4 and TXNIP in both groups were significantly lower than those in the HG group(P<0.05,P<0.01),and the RECs activity,migration number,tube formation number,and the expression of NOX4 and TXNIP in the HG+H400 group were further significantly decreased compared with those in the HG+H100 group(P<0.01).CONCLU-SION Hyperoside could significantly inhibit the high glucose-induced excessive proliferation of RECs.The mechanism may be relat-ed to the inhibition of NOX4/TXNIP activation in high-glucose environment.关键词
金丝桃苷/高葡萄糖/视网膜内皮细胞/过度增殖/NOX4/TXNIPKey words
hyperoside/high glucose/retinal endothelial cells/excessive proliferation/NOX4/TXNIP分类
医药卫生引用本文复制引用
赵越,陈娟,王高祥,吴豪,余旭,孙心怡,缪鋆鋆,周雷,施荣伟,周希乔..金丝桃苷对高糖诱导视网膜内皮细胞过度增殖的抑制作用[J].南京中医药大学学报,2025,41(8):1047-1054,8.基金项目
江苏省中医药科技发展计划重点项目(ZD202406) （ZD202406）
国家自然科学基金青年科学基金项目(82405172) （82405172）
江苏省基础研究专项资金(软科学研究)专项(BK20241153) （软科学研究）
江苏省中医院"优秀青年博士培养计划"(2024QB022) （2024QB022）
克孜勒苏柯尔克孜自治州科技局基础研究项目(2025-06) （2025-06）
