南京中医药大学学报2025，Vol.41Issue(8)：1066-1074,9.DOI:10.14148/j.issn.1672-0482.2025.1066
基于不同提取方式及指标成分测定的甘肃黄芪饮片质量评价研究
Study on the Quality Evaluation of Astragalus Membranaceus Pieces in Gansu Based on Different Extraction Methods and In-dex Component Determination
摘要
Abstract
OBJECTIVE To establish a method for determining the content of multiple components in Astragalus membranaceus,compare the content differences of Astragalus membranaceus in different regions and with different extraction methods in Gansu,and e-valuate the quality of Astragalus membranaceus from different origins of Gansu.METHODS Astragalus membranaceus samples from 40 origins in Gansu were collected on site.The content of Astragaloside Ⅳ,Calycosin-7-O-β-D-glucopyranoside,Ononin,Form-ononetin and Calycosin was determined by HPLC external standard method,and the quality differences of Astragalus membranaceus from 40 origins of Gansu were analyzed by combining bar graphs and line graphs.RESULTS Weiyuan County and Min County of Dingxi City showed high Astragaloside Ⅳ content in both extraction methods,indicating that the Astragalus membranaceus in these two regions has advantages in Astragaloside Ⅳ as an active component;for Calycosin-7-O-β-D-glucopyranoside and Ononin,although the performance of different regions in different extraction methods was different,in general,Weiyuan County and Min County of Dingxi City and Longnan City showed high content in both extraction methods.Water extraction method was more conducive to the extraction of Calycosin-7-O-β-D-glucopyranoside and Ononin,while alcohol extraction method was more conducive to the extraction of Astragalo-side Ⅳ,Calycosin and Ononin.CONCLUSION The content of chemical components of Astragalus membranaceus from different ori-gins is quite different,and the choice of extraction method has a significant effect on the content of active components,while the change trend of different compounds in the two extraction methods is basically the same.The established HPLC multi-component content de-termination method of Astragalus membranaceus is stable and reliable,which can provide a scientific basis for the quality control and comprehensive evaluation of Astragalus membranaceus medicinal materials.关键词
黄芪/质量评价/HPLC/毛蕊异黄酮葡萄糖苷/黄芪甲苷:标准汤剂Key words
Astragalus membranaceus/quality evaluation/HPLC/Calycosin-7-O-β-D-glucopyranoside/Astragaloside Ⅳ:standard decoction分类
医药卫生引用本文复制引用
周珂,蔡文韬,朱慧敏,倪亮,张先林,姚毅,刘史佳..基于不同提取方式及指标成分测定的甘肃黄芪饮片质量评价研究[J].南京中医药大学学报,2025,41(8):1066-1074,9.基金项目
国家老药工传承工作室建设项目(国中医药人教函[2024]255号) （国中医药人教函[2024]255号）
江苏省老药工传承工作室建设项目(苏中医科教[2024]4号) （苏中医科教[2024]4号）
贵州同德药业科技项目(TDKY202302) （TDKY202302）
南京市中医药传承创新融创平台项目 （）
