山东农业大学学报（自然科学版）2025，Vol.56Issue(4)：670-680,11.DOI:10.3969/j.issn.1000-2324.2025.04.013
高产单宁酶的黑曲霉菌株鉴定及其固态发酵产酶条件优化
Identification of High Tannase-producing Aspergillus niger Strains and Optimization of Solid-state Fermentation Conditions for Enzyme Production
摘要
Abstract
This study isolates and screens a fungal strain from tea leaves using a tannase identification medium.The strain is identified as Aspergillus niger through morphological characteristics and ITS sequence analysis,and designated as strain ALF11-1.Fermented with the spore suspension of strain ALF11-1,the tannase production activity of black tea leaf stalks reaches 10.42 U/mL.Subsequently,the optimal fermentation conditions are determined through one-factor and response surface experiments,which are as follows:temperature 31℃,incubation time 4 d,inoculum size 4%,and ammonium chloride concentration 7%.Under these conditions,the predicted enzyme activity is 24.9 U/mL,while the measured activity reaches 25.92 U/mL.Compared to the pre-optimization enzyme activity(10.4 U/mL),the optimized solid-state fermentation conditions enhance tannase production by strain ALF11-1 approximately 2.49-fold.关键词
茶树/单宁酶/黑曲霉/响应面Key words
Tea plant/tannase/Aspergillus niger/response surface分类
化学化工引用本文复制引用
王薪喻,李海鹏,宋大鹏,孙霞,王鲲鹏,房峰祥,丁仕波,杨峰山..高产单宁酶的黑曲霉菌株鉴定及其固态发酵产酶条件优化[J].山东农业大学学报（自然科学版）,2025,56(4):670-680,11.基金项目
国家现代农业产业技术体系项目(CARS-19) （CARS-19）
黑龙江省"双一流"学科协同创新成果项目(LJGXCG2023-036) （LJGXCG2023-036）
