南阳师范学院学报2025，Vol.24Issue(5)：37-43,7.
基于双足DNA Walker的生物传感器用于人脱嘌呤/嘧啶核酸内切酶1高灵敏检测
Bipedal DNA Walker based Biosensor for high-sensitive detection of Apurinic/Apyrimidinic Endonuclease 1
摘要
Abstract
Human apurinic/apyrimidinic endonuclease 1(APE1),as a crucial enzyme within the base excision repair(BER)pathway,exhibits abnormal expression in tumor cells and serves as one of the biomarkers for cancer diagnosis.Therefore,the development of highly sensitive methods for detecting APE1 activity holds significant research value.This study,based on the high walking efficiency and stability of the bipedal DNA walker,utili-zes exonuclease Ⅲ(Exo Ⅲ)as the driving force and gold nanoparticles(AuNPs)as the carriers of the track strands.Leveraging the fluorescence quenching mechanism,a bipedal DNA walker fluorescence biosensor for the highly sensitive detection of APE1 has been developed.Under optimal detection conditions,the activity of APE1 exhibited a favorable linear relationship with the fluorescence intensity of the system within the range of 0.01 U/mL to 0.5 U/mL.The linear equation was determined to be F=173.00CAPE1+99.45,with a detection limit of 0.006 U/mL.This method can be applied to detect APE1 activity in serum-mimicking samples,with a spike re-covery rate ranging from 96.4%to 98.5%.关键词
APE1/金纳米颗粒/双足 DNA Walker/信号放大/生物传感器Key words
human apurinic/apyrimidinic endonuclease1/gold nanoparticles/bipedal DNA walker/signal am-plification/biosensors分类
化学化工引用本文复制引用
雷鸣琳,孟雪莹,卢宏飞,孟雪明,赵艺卓,邢小静..基于双足DNA Walker的生物传感器用于人脱嘌呤/嘧啶核酸内切酶1高灵敏检测[J].南阳师范学院学报,2025,24(5):37-43,7.基金项目
河南省科技攻关项目(252102320248) （252102320248）
河南省青年骨干教师培育基金(2021GGJS123) （2021GGJS123）
河南省高等学校重点科研项目(24A150027) （24A150027）
