青岛大学学报（自然科学版）2025，Vol.38Issue(3)：15-22,34,9.DOI:10.3969/j.issn.1006-1037.2025.03.03
双链DNA胞苷脱氨酶检测DNA甲基化
Double-stranded DNA Cytosine Deaminase Detects DNA Methylation
摘要
Abstract
A highly sensitive double stranded DNA cytosine deaminase DddA coupling methylation-specific PCR method was developed for detecting DNA methylation,avoiding DNA sample degradation in bisulfite conversion coupled Methylation-Specific PCR.In Dd-dA conversion-based methylation specific real-time quantitative PCR,the DddA deamina-ted the DNA samples firstly,then performed the real-time PCR with methylation specific primers.According to standard curve of the target genes,the methylation level of the DNA sample could be evaluated.The results showed that the DddA can discriminate the methylate with unmethylated DNA well.And DddA-qMSP can effectively detect the methylation status of colorectal cancer marker genes SEPT9 and SDC2.关键词
双链DNA/双链DNA胞嘧啶脱氨酶/胞嘧啶甲基化/表观遗传/基因检测Key words
double-stranded DNA/double-stranded DNA cytosine deaminase/cytosine methylation/epigenetic inheritance/genetic testing分类
医药卫生引用本文复制引用
唐悦,李新敏..双链DNA胞苷脱氨酶检测DNA甲基化[J].青岛大学学报（自然科学版）,2025,38(3):15-22,34,9.基金项目
国家自然科学基金(批准号:RZ2000004256)资助. （批准号:RZ2000004256）
