生态学杂志2025，Vol.44Issue(8)：2541-2547,7.DOI:10.13292/j.1000-4890.202508.032
江西省旱作和水田土壤氧化亚氮还原菌丰度及其主要影响因素
Abundance of nitrous oxide reducers and the driving factors in dryland and paddy soils from Jiangxi Pro-vince
摘要
Abstract
To explore the characteristics of nitrous oxide-reducing bacteria abundance in dryland and paddy soils and their influencing factors,real-time quantitative PCR was used to measure the abundance of nosZ Ⅰ and nosZ Ⅱgenes in the soils of dryland peanut and flooded paddy fields from 16 sites in Jiangxi Province.The main influencing factors were analyzed.The results showed that the copy number of the nosZ Ⅱ gene in both dryland and paddy soils was significantly higher than that of nosZ Ⅰ,indicating that nosZ Ⅱ-type nitrous oxide-reducing bacteria dominated in acidic agricultural soils.The abundance of nosZ Ⅰ in dryland soils was 2.28×108 copies·g-1,significantly high-er than that found in paddy soils(1.33×108 copies·g-1),indicating that nosZ Ⅰ-type nitrous oxide-reducing bac-teria may prefer dryland soils over paddy soils.However,there was no significant difference in the abundance of nosZ Ⅱ genes between dryland and paddy soils.In dryland soils,the total carbon content was the most important factor influencing the copy numbers of nosZ Ⅰ and nosZ Ⅱ genes,possibly because nitrous oxide-reducing bacteria are heterotrophic microorganisms with total carbon as the carbon sources and energy.Meanwhile,available phos-phorus content and pH value were the most important factors explaining the variations of copy numbers of nosZ Ⅰand nosZ Ⅱ genes in paddy soils,likely because the total carbon content in paddy soils is high and not a limiting factor,making phosphorus and pH more influential.In summary,the abundance of nitrous oxide-reducing bacteria differed in dryland peanut and paddy soils in Jiangxi Province,with nosZ Ⅰ-type nitrous oxide-reducing bacteria fa-voring dryland soils and nosZ Ⅱ showing no significant preference.关键词
土地利用方式/氮循环/氧化亚氮/nosZ Ⅰ/nosZ ⅡKey words
land use pattern/nitrogen cycle/nitrous oxide/nosZ Ⅰ/nosZ Ⅱ引用本文复制引用
成于恒,冯蒙蒙,宛颂,邓米林,贺纪正,林永新..江西省旱作和水田土壤氧化亚氮还原菌丰度及其主要影响因素[J].生态学杂志,2025,44(8):2541-2547,7.基金项目
国家自然科学基金项目(42077041,41930756)资助. （42077041,41930756）
