烟草科技2025，Vol.58Issue(8)：47-58,12.DOI:10.16135/j.issn1002-0861.2024.0649
海南雪茄烟叶晾制干筋期主要致霉菌分离鉴定及对烟叶挥发性成分的影响
Isolation and identification of main types of molds in Hainan cigar tobacco at stem-drying stage during air-curing and their influence on leaf volatile components
摘要
Abstract
To evaluate the types of molds and main molds on cigar tobacco leaves from different production areas in Hainan Province at the stem-drying stage during air-curing,plate counting method was used to compare the amounts of molds on moldy tobacco leaves from five planting areas(Danzhou,Ledong,Tunchang,Dongfang and Wuzhishan).The molds were identified through morphology and ITS sequencing,and the effect of temperature on their growth was investigated.By artificially inoculating the molds on tobacco leaves without mold,the main mildew-causing fungi were determined according to the colonization of the strains,and the effects of the molds on the volatile components in the leaves were analyzed by headspace-solid-phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS).The results showed that:1)There were differences in the mold species on the moldy leaves from these areas.Aspergillus chevalieri was detected in the moldy samples from Tunchang,Dongfang and Wuzhishan,accounting for 99％,90％and 90％of the total mold amount,respectively.Penicillium citrinum was found in the moldy samples from Danzhou and Ledong,accounting for 60％and 45％of the total mold amount,respectively.Penicillium oxalicum only existed in the Danzhou sample,accounting for 20％;Aspergillus occultus appeared in the Ledong sample,accounting for 50％.2)The growth of the four main mold strains was inhibited at 37 ℃.3)The Aspergillus occultus strain had a strong ability to cause mildew and tended to cause mold on Hainan cigar tobacco leaves.4)The contents(mass fractions)of esters in Hainan cigar tobacco leaves increased significantly after molding,and methyl hexadecanoate,methyl pentadecanate,methyl stearate and methyl 9,12-octadecadienoate could be used as markers of cigar tobacco leaf mold.关键词
雪茄烟叶/晾制/致霉性/霉菌/挥发性成分Key words
Cigar tobacco/Air-curing/Mildew-causing property/Mildew/Volatile component分类
轻工纺织引用本文复制引用
谢求璇,张鸽,黄阔,郜晓妍,蔡斌,颜统晶,叶长文,李栋,丁伟,胡清源..海南雪茄烟叶晾制干筋期主要致霉菌分离鉴定及对烟叶挥发性成分的影响[J].烟草科技,2025,58(8):47-58,12.基金项目
中国烟草总公司重大科技项目"手工雪茄烟加工过程中霉变生防控制技术研究与应用"[11202201034(XJ-05)] （XJ-05）
中国烟草总公司郑州烟草研究院青年人才托举工程计划项目"手工雪茄烟霉变拮抗微生物的筛选和应用研究"(702021CR0090) （702021CR0090）
河南省博士后科研资助项目"生物防治雪茄烟叶霉变的作用机理及效果强化研究"HN2024187(702024AS0350). （702024AS0350）
