中国兽医科学2025，Vol.55Issue(8)：1122-1130,9.DOI:10.16656/j.issn.1673-4696.2025.0140
黑果枸杞花青素通过介导Nrf2和NF-κB通路缓解低氧诱导的H9c2心肌细胞氧化应激
Anthocyanin of Lycium barbarum attenuate hypoxia-induced oxidative stress in H9c2 cardiomyocytes via the Nrf2 and NF-κB signaling pathways
摘要
Abstract
To investigate the underlying molecular mechanisms of anthocyanin of Lycium barbarum(AC)on hypoxia-induced oxidative stress in H9c2 cardiomyocytes and their alleviating effects.A hypoxic H9c2 cardiomyocyte model was established under 1％O2,the mRNA and protein expression levels of key factors in the Nrf2 and NF-κB signaling pathways were analyzed using qRT-PCR and Western-blot under normoxic and hypoxic conditions following AC treatment.The results demonstrated that hypoxia signifi-cantly(P＜0.05)reduced mRNA expression of Nrf2 pathway components(KAP1,Nrf2,HO-1,and NQO1),with Nrf2 and KAP1 protein levels showing extremely significant decreases(P＜0.01),while HO-1 andNQO1 proteins were significantly downregulated(P＜0.05).Compared to the hypoxia group,the hypoxia+AC group exhibited extremely significant upregulation of both mRNA and protein levels of KAP1,Nrf2,HO-1,and NQO1(P＜0.01).Hypoxia induced extremely significant(P＜0.01)increases in mRNA and protein expression of NF-κB pathway components(IKK-α,IKK-β,NF-κB,TNF-α,and COX-2).AC treatment under hypoxia significantly suppressed these increases(P＜0.01).Further experiments using the NF-κB activator imiquimod revealed that the hypoxia+imiquimod group exhibited extremely significant upregulation of IKK-β and NF-κB proteins(P＜0.01)and significant increases in IKK-α,TNF-α,and COX-2 proteins(P＜0.05).In contrast,the hypoxia+AC+imiquimod group showed extremely significant downregulation of all these proteins(P＜0.01).These findings indicate that AC alleviates hypoxia-induced oxidative stress in H9c2 cardiomyocytes by activating the Nrf2 pathway and suppressing the NF-κB signaling pathway,thereby providing cardiomyocyte protection.关键词
花青素/低氧/H9c2心肌细胞/氧化应激/Nrf2通路/NF-κB通路Key words
anthocyanin/hypoxia/H9c2 cardiomyocytes/oxidative stress/Nrf2 pathway/NF-κB pathway分类
农业科技引用本文复制引用
许恩榕,吴华,沈童,武小庆,陈林,郑润韬,尹宇欣,宋致欣,段馨儿..黑果枸杞花青素通过介导Nrf2和NF-κB通路缓解低氧诱导的H9c2心肌细胞氧化应激[J].中国兽医科学,2025,55(8):1122-1130,9.基金项目
青海省科技计划应用基础研究面上资助项目(2022-ZJ-927) （2022-ZJ-927）
