中国兽医科学2025，Vol.55Issue(8)：995-1002,8.DOI:10.16656/j.issn.1673-4696.2025.0146
L蛋白第177位氨基酸突变对O型口蹄疫病毒生物学特性的影响
Impact of amino acid mutation at position 177 in the L protein on the biological characteristics of O-type foot-and-mouth disease virus
摘要
Abstract
To investigate the impact of amino acid mutations in the leader protein(Lpro)on the bio-logical characteristics of foot-and-mouth disease virus(FMDV),a full-length cDNA clone of FMDV con-taining the L-V177M point mutation was constructed by FMDV reverse genetics technology.The construct was transfected into IBRS-2 cells for in vivo rescue.The effects of the mutated amino acid on the bio-logical properties of FMDV were assessed through growth curves,PCR Array analysis,and 146S antigen production assays.The results indicated that while the replication dynamics of the point-mutated virus rO-L/V177M were similar to those of the parental virus rWT in IBRS-2 cells,the former exhibited significantly reduced viral titers and formed smaller plaque.Additionally,the expression levels of antiviral genes OAS2,NOS2,IFITM1,and pro-inflammatory genes CCL4,PYCARD,CXCL11,and CD80 were sig-nificantly upregulated in the mutant.However,there was no significant difference in antigen produc-tion between the two viruses in suspended BHK-21 cells.This study elucidates the critical role of this amino acid site in FMDV replication and pathogenicity,provides new insights into the interaction be-tween FMDV and the host immune system,and offers a potential candidate strain for the development of live attenuated vaccines.The findings not only deepen the understanding of the function of the FMDV Lpro protein but also provide important theoretical support for FMD vaccine development and the development of targeted antiviral strategies.关键词
口蹄疫病毒/L蛋白/生物学特性Key words
foot-and-mouth disease virus/leader protein/biological characteristics分类
农业科技引用本文复制引用
李静雯,曹伟军,杨帆,朱紫祥,张伟,郑海学,蒋大伟..L蛋白第177位氨基酸突变对O型口蹄疫病毒生物学特性的影响[J].中国兽医科学,2025,55(8):995-1002,8.基金项目
国家重点研发计划项目(2021YFD1800302) （2021YFD1800302）
国家生猪产业技术体系项目(CARS-35,CARS-39-13) （CARS-35,CARS-39-13）
甘肃省自然科学基金项目(23JRRA547) （23JRRA547）
