中国兽医科学2025，Vol.55Issue(8)：1003-1010,8.DOI:10.16656/j.issn.1673-4696.2025.0145
O型口蹄疫病毒样颗粒磷酸钙仿生矿化的工艺优化
Process optimization of bionic mineralization of calcium phosphate in virus-like particles of foot-and-mouth disease serotype O
摘要
Abstract
Biomimetic mineralization of calcium phosphate can effectively improve the thermal stability and immunogenicity of the foot-and-mouth disease virus-like particles(FMD VLPs)vaccine.In order to promote calcium phosphate mineralized FMD VLPs to industrial production,this study optimized the reaction system based on the existing mineralization process,including ion types and concentra-tions,reaction system temperature,and reaction speed.The influence of these factors on the minerali-zation of FMD VLPs phosphate-based biomimetic mineralization was determined based on the mineralization rate,morphology,and size of the mineralized products.Finally,we selected the optimal mineralization protocol based on the experimental results.Among them,the optimal reaction system was 16.7 mmol/L Ca(NO3)2,0.3 mmol/L NaH2PO4,and 1.2 mmol/L Na2HPO4.The reaction temperature can be raised to 25 ℃,and the stirring speed can be reduced to 1 500 r/min.Finally,the optimized process was scaled up proportionally to 100 mL for validation,and the results showed that scaling up the reaction system proportionally could still ensure good mineralization efficiency and form uniformly shaped mineralized foot-and-mouth disease virus-like particles.关键词
口蹄疫病毒样颗粒/磷酸钙仿生矿化/工艺优化Key words
foot-and-mouth disease virus-like particles/bionic mineralization of calcium phos-phate/process optimization分类
农业科技引用本文复制引用
尉嘉锡,王翱飞,张铭洋,郭伟,刘瑞鹏,郭慧琛,孙世琪,周静静,曾小英..O型口蹄疫病毒样颗粒磷酸钙仿生矿化的工艺优化[J].中国兽医科学,2025,55(8):1003-1010,8.基金项目
国家重点研发计划项目(2023YFD1800300) （2023YFD1800300）
国家自然科学基金项目(32301127) （32301127）
兰州市人才创业创新项目(2023-RC-3) （2023-RC-3）
甘肃省重大科技专项(24ZDWA004) （24ZDWA004）
宁夏重点研发计划项目(2024BBF02017) （2024BBF02017）
