中国兽医科学2025，Vol.55Issue(8)：1033-1040,8.DOI:10.16656/j.issn.1673-4696.2025.0131
核糖体蛋白S6激酶在卵形巴贝斯虫侵染长角血蜱过程中的作用
Role of Ribosomal protein S6 kinase in the infection of Haemaphysalis longicornis with Babesia ovata
摘要
Abstract
Babesia ovata is a pear-shaped parasite that resides in the blood of cattle and can cause ovine babesiosis in infected livestock.Haemaphysalis longicornis is its primary biological vector.Ribosomal protein S6 kinase(RpS6K),as an important cellular signaling molecule in ticks,plays a crucial role in the process of pathogen infection.The full-length sequence of RpS6K gene from Haemaphysalis longicornis was amplified by PCR and designed small interfering RNA(siRNA)for RpS6K based on the obtained sequence.Experimental cattle were inoculated with Babesia ovata via the jugular vein to establish an animal infection model.Expression of the RpS6K gene was inhibited using siRNA,and the physiological data of RpS6K during Haemaphysalis longicornis infection with Babesia ovalis were analyzed by ANOVA.The results showed that the full length of the RpS6K gene in Haemaphysalis longicornis was 2 922 bp.The inhibition experiment reduced the expression abundance of the RpS6K gene to 31.54％(P＜0.05).Animal infection tests indicated that the infection rate of Babesia ovata in the experimental group decreased significantly compared with the control group.Interfering with the RpS6K gene in Haemaphysalis longi-cornis can effectively block the vertical transmission of Babesia ovata in tick,thus providing a new direction for the prevention and control of babesiosis.关键词
长角血蜱/卵形巴贝斯虫/核糖体蛋白S6激酶/RNAiKey words
Haemaphysalis longicornis/Babesia ovata/ribosomal protein S6 kinase/small inter-fering RNA分类
农业科技引用本文复制引用
车丽妍,林向鑫,郑立东,常国勤,李星,王希伟,樊亮,殷宏,罗建勋,刘光远,罗金,高仙,张高峰,赵帅阳,任巧云,胡东生,李元飞,曹立华..核糖体蛋白S6激酶在卵形巴贝斯虫侵染长角血蜱过程中的作用[J].中国兽医科学,2025,55(8):1033-1040,8.基金项目
甘肃省自然科学基金项目(25JRRA437) （25JRRA437）
国家肉牛牦牛产业技术体系项目(CARS-37) （CARS-37）
中国农业科学院科技创新工程项目(CAAS-ASTIP-2021-LVRI) （CAAS-ASTIP-2021-LVRI）
国家寄生虫资源库项目(NPRC-2019-194-30) （NPRC-2019-194-30）
