中国药物经济学2025，Vol.20Issue(8)：48-53,6.DOI:10.12010/j.issn.1673-5846.2025.08.009
甲胎蛋白调控HIPPO信号通路对肝细胞癌细胞重编程基因表达的影响
Effect of Alpha-fetoprotein Regulating the HIPPO Signaling Pathway on the Expression of Reprogramming Genes in Hepatocellular Carcinoma Cells
摘要
Abstract
Objective To explore the effect of alpha-fetoprotein(AFP)regulating the HIPPO signaling pathway on the expression of reprogramming genes in hepatocellular carcinoma(HCC)cells.Methods Construct the HUH7 cell line with stable down-regulation of AFP and the HLE cell line with stable up-regulation of AFP According to the different transfected viruses,they were divided into the HUH7 group(untransfected),the HUH7C group(transfected with LV-NC),the HUH7-SIAFP group(transfected with LV-siAFP),HLE(untransfected),HLEC(transfected with LV-NC),and HLE-AFP(transfected with LV-AFP).The effect of AFP on the expression of reprogramming genes was detected by western bloting.The protein interaction between AFP and YAP was simulated by Discovery studio software.The correlation between YAP and reprogramming genes was analyzed by GEPIA2 online database.The effect of down-regulation or up-regulation of AFP on the colony formation of HCC cells was detected by plate cloning.The effect of down-regulation or up-regulation of AFP on the distribution of YAP protein in the cytoplasm and nucleus of HCC cells was observed by immunofluorescence assay.The interaction between AFP and YAP protein in HUH7 cells was detected by CO-IP assay.Results Upregulation of AFP can promote the expression of reprogramming genes in HCC cells,while downregulation of AFP can inhibit the expression of reprogramming genes in HCC cells.GEPIA2 online database analysis showed a positive correlation between YAP and reprogramming gene table.Discovery studio software simulation,CO-IP experiment,and immunofluorescence experiment suggested that AFP and YAP interact,and AFP can affect the nuclear localization of YAP.Conclusion In HCC cells,AFP may enhance the expression of downstream reprogramming genes by promoting the aggregation of YAP in the HIPPO signaling pathway in the nucleus.关键词
甲胎蛋白/肝癌/HIPPO/重编程基因Key words
Alpha-fetoprotein/Liver cancer/HIPPO/Reprogramming genes分类
医药卫生引用本文复制引用
王照良..甲胎蛋白调控HIPPO信号通路对肝细胞癌细胞重编程基因表达的影响[J].中国药物经济学,2025,20(8):48-53,6.基金项目
海南省卫生健康行业科研项目(22A200221) （22A200221）
海南省临床医学中心建设项目资助(琼卫医函[2022]341号) （琼卫医函[2022]341号）
