种子2025，Vol.44Issue(7)：234-239,245,7.DOI:10.16590/j.cnki.1001-4705.2025.07.234
紫荆花粉萌发液体培养基的筛选及培养条件优化
Screening of Liquid Medium for in Vitro Germination and Optimization of Culture Conditions of Cercis chinensis Pollen
摘要
Abstract
In order to screen out the most suitable components of liquid culture medium of Cercis chinensis,the pollen of Cercis chinensis was used as the experimental material,and the liquid culture method was adopted to explore the effects of sucrose,boric acid,calcium nitrate,different culture temperatures and times on the germination of Cercis chinensis pollen.The results showed that the suitable medium components for pollen in vitro germination were:20%sucrose+200 mg/L boric acid+100 mg/L calcium nitrate.At this time,the pollen germination rate reached 86.43%,and the length of the pollen tube was 175.37μm.The appropriate in vitro culture time was 5 hours and the culture temperature was 25℃.Temperature was a key influencing factor.Unsuitable temperature could lead to a decrease in the activity of protective enzymes,disrupt the balance of reactive oxygen species me-tabolism,intensify membrane lipid peroxidation,and hinder pollen germination and pollen tube growth.关键词
紫荆/液体培养基/花粉萌发/花粉管长度/培养温度Key words
Cercis chinensis/liquid medium/pollen germination/pollen tube length/culture temperature分类
农业科技引用本文复制引用
杨延红,陈文双,姜君军,马晶晶,李慧敏,裴宝智,姜倩倩..紫荆花粉萌发液体培养基的筛选及培养条件优化[J].种子,2025,44(7):234-239,245,7.基金项目
国家自然科学基金(31301733) （31301733）
山东省高等学校科技发展计划(J18KA157) （J18KA157）
山东省大学生创新创业训练项目(S202411067018) （S202411067018）
