中国实验动物学报2025，Vol.33Issue(7)：935-946,12.DOI:10.3969/j.issn.1005-4847.2025.07.001
MMTV-PyMT乳腺癌小鼠不同周龄肿瘤微环境中免疫细胞的组成变化
Changes in the composition of immune cells in the tumor microenvironment of MMTV-PyMT breast cancer mice at different ages
摘要
Abstract
Objective This study aimed to investigate the tumorigenic properties of MMTV-PyMT breast cancer transgenic mice at different ages(in weeks)and the changes in the composition of immune cells in the tumor microenvironment.Methods Eight groups of 4,6,8,10,12,14,16 and 18 weeks of age MMTV-PyMT female mice(FVB mice as the background)and one group of 8 weeks of FVB female mice were prepared for routine blood testing,the pathological changes of the mammary gland and lung metastases were observed by histopathological sections,and the immune cells in blood,spleen,and tumor were analyzed by flow cytometry.Results MMTV-PyMT mice showed adenular ductal lesions at 4～6 weeks of age;the ductal portion expanded to the growth boundary at 8～9 weeks of age,and then gradually broke through the glandular boundary to form early breast cancer at 8～12 weeks of age,and advanced breast cancer at 10～14 weeks of age.At 12 weeks of age,metastases were visible in the lungs of some mice,and at 14 weeks of age,the number of metastases in the lungs increased significantly.As the age of the mice increased,the number of white blood cells,neutrophils,and platelets in their blood increased gradually,while the lymphocytes and erythrocytes showed a gradual downward trend.Flow cytometry showed that with the increase in age,the proportion of T cells in the spleen and tumor gradually decreased,the MDSCs in the blood,spleen,and tumor gradually increased,and the NK cells in the tumor also gradually increased.Conclusions This study analyzed routine blood tests,pathology,and immune cells in the tissues of MMTV-PyMT mouse models of different weeks of age,providing a novel perspective on the dynamic alterations of the tumor immune microenvironment during the malignant progression of breast cancer.关键词
MMTV-PyMT转基因小鼠/乳腺癌/肺转移/肿瘤微环境Key words
MMTV-PyMT transgenic mice/breast cancer/lung metastases/tumor microenvironment分类
生物科学引用本文复制引用
林晓晨,龚丽红,郭迎雪,金璐,陈梦芸,王鹏皓,余翠,傅惠英,寿旗扬..MMTV-PyMT乳腺癌小鼠不同周龄肿瘤微环境中免疫细胞的组成变化[J].中国实验动物学报,2025,33(7):935-946,12.基金项目
国家自然科学基金(82274175,82174026),国家中医药管理局科技司-浙江省中医药管理局共建科技计划项目(GZY-ZJ-KJ-23069).Funded by National Natural Science Foundation of China(82274175,82174026),Science and Technology Department of State Administration of Traditional Chinese Medicine-Zhejiang Provincial Administration of Traditional Chinese Medicine Co-Construction Project(GZY-ZJ-KJ-23069). （82274175,82174026）
