中国实验动物学报2025，Vol.33Issue(7)：980-989,10.DOI:10.3969/j.issn.1005-4847.2025.07.005
不同剂量IDPN对多发性抽动症小鼠的影响
Effect of different doses of 3,3'-iminodipropionitrile in Tourette syndrome mice
摘要
Abstract
Objective To investigate the effects of different doses of 3,3'-iminodipropionitrile(IDPN)in mice with Tourette syndrome(TS)to optimize the dosage and establish a stable TS model.Methods Thirty-two male C57BL/6J mice were divided randomly into a control group and a model group.The model group was further subdivided and administered low-dose(300 mg/kg),medium-dose(350 mg/kg),and high-dose(400 mg/kg)IDPN,respectively,while the control group received an equal volume of saline by intraperitoneal injection for 7 days.Modeling effectiveness was assessed on days 0 and 7 using stereotypy scoring,the number of head and body twitches,and open-field testing.Dopamine and tumor necrosis factor(TNF-α)levels in serum and brain tissue were measured by enzyme-linked immunosorbent assay.The morphology of striatum and hippocampus tissues were observed by hematoxylin/eosin(HE)staining.Results The stereotypy scores indicated successful modeling of TS in the medium-and high-dose groups.Significant behavioral changes in the open-field test were only detected in the high-dose IDPN group(P＜0.05).Serum dopamine levels were significantly increased(P＜0.05)in the model group,and TNF-α levels were significantly elevated in the medium-and high-dose groups(P＜0.05),but there was no significant difference in brain-tissue levels(P＞0.05).HE staining showed that the neurons and glial cells in the striatum and hippocampus were morphologically normal in the control group,but there were some neurodegenerative changes and a few swollen neuronal cell bodies in the striatum and hippocampus in the model group,and obvious lymphocyte infiltration in the striatum and hippocampus in the high-dose group.Conclusions Through systematic comparison of varying IDPN dosages in establishing a TS model,this study identified 400 mg/kg as the optimal dosage for effective model induction.These findings provide data to support dose optimization in the TS model and offer valuable references for ensuring the smooth progress of early-stage experiments,which could aid the evaluation of the therapeutic effects of subsequent drug interventions.关键词
抽动症/3,3'-亚氨基二丙腈/多巴胺/肿瘤坏死因子-α/益生菌Key words
Tourette syndrome/3,3'-iminodipropionitrile/dopamine/tumor necrosis factor-α/probiotic分类
生物科学引用本文复制引用
乔嘉祺,何秋雯,张文羿..不同剂量IDPN对多发性抽动症小鼠的影响[J].中国实验动物学报,2025,33(7):980-989,10.基金项目
内蒙古自治区高等学校创新团队发展计划(NMGIRT2411),中央引导地方科技发展资金(2024ZY0168).Funded by Inner Mongolia Autonomous Region University Innovation Team Development Program(NMGIRT2411),Central Guidance for Local Scientific and Technological Development Funds(2024ZY0168). （NMGIRT2411）
