中国实验动物学报2025，Vol.33Issue(7)：1021-1031,11.DOI:10.3969/j.issn.1005-4847.2025.07.009
基于肠道菌群探讨GS-9620改善IMQ诱导的小鼠银屑病样炎症的研究
Effect of GS-9620 in imiquimod-induced psoriasis-like inflammation in mice based on gut microbiota
摘要
Abstract
Objective To explore the mechanism of GS-9620 in improving imiquimod(IMQ)-induced psoriasis-like inflammation by regulating the Th1/Th17-related immune response,and to investigate its regulatory effect on the gut microbiota in mice.Methods An IMQ-induced psoriasis-like inflammation model was established in BALB/c mice.The severity of the skin lesions was evaluated by psoriasis area and severity index(PASI)score.The proportions of CD4+interleukin(IL)-17+and CD4+interferon(IFN)-γ+cells in spleen tissue were detected by flow cytometry.Levels of the inflammatory factors tumor necrosis factor(TNF)-α,IL-1β,and IL-6 in skin tissues were determined by enzyme-linked immunosorbent assay,and pathological analysis was performed by hematoxylin/eosin staining.The effects of GS-9620 on the structure of the gut microbiota in control,IMQ model,and GS-9620-treated mice were detected by 16S rRNA sequencing.Results GS-9620 significantly reduced the PASI score in IMQ-induced mice and effectively reduced the proportions of CD4+IL-17+and CD4+IFN-γ+cells in the spleen.GS-9620 also significantly down-regulated the expression levels of TNF-α,IL-1β,and IL-6 in skin tissues.16S rRNA sequencing showed that GS-9620 significantly regulated the abundance of gut microbiota related to inflammation,including the relative abundances of bacteria such as Lachnospiraceae_NK4A136_group,Lachnospiraceae_UCG-008,Alloprevotella,Desulfovibrio,Prevotellaceae_UCG-001,and Alistipes.Conclusions GS-9620 effectively alleviates IMQ-induced psoriasis-like skin inflammation in mice by regulating the expression of Th1/Th17-related inflammatory factors.It may also improve IMQ-induced clinical symptoms by regulating the structure of the gut microbiota,thus providing a new theoretical basis for the treatment of psoriasis.The result of this study provide important experimental evidence to support further investigations into the application of GS-9620 for the treatment of psoriasis.关键词
银屑病/银屑病样炎症小鼠模型/肠道菌群/GS-9620/16S rRNAKey words
psoriasis/psoriasis-like inflammation animal models/gut microbiota/GS-9620/16S rRNA分类
生物科学引用本文复制引用
杨婧宇,张倩,陈思,王晓堂,宋国华..基于肠道菌群探讨GS-9620改善IMQ诱导的小鼠银屑病样炎症的研究[J].中国实验动物学报,2025,33(7):1021-1031,11.基金项目
深圳市科技研发资金(JCYJ20220530141615035),实验动物新资源创制与利用山西省科技创新人才团队(202204051002032).Funded by Shenzhen Science and Technology Research and Development Fund(JCYJ20220530141615035),Shanxi Provincial Science and Technology Innovation Team for the Creation and Utilization of New Resources of Laboratory Animals(202204051002032). （JCYJ20220530141615035）
