首页|期刊导航|中国结合医学杂志（英文版）|Suppression of Hepatocellular Carcinoma through Apoptosis Induction by Total Alkaloids of Gelsemium elegans Benth.
中国结合医学杂志（英文版）2025，Vol.31Issue(9)：792-801,10.DOI:10.1007/s11655-025-4121-5
Suppression of Hepatocellular Carcinoma through Apoptosis Induction by Total Alkaloids of Gelsemium elegans Benth.
Suppression of Hepatocellular Carcinoma through Apoptosis Induction by Total Alkaloids of Gelsemium elegans Benth
摘要
Abstract
Objective:To evaluate the anti-hepatocellular carcinoma(HCC)activity of total alkaloids from Gelsemium elegans Benth.(TAG)in vivo and in vitro and to elucidate their potential mechanisms of action through transcriptomic analysis.Methods:TAG extraction was conducted,and the primary components were quantified using high-performance liquid chromatography(HPLC).The effects of TAG(100,150,and 200 μg/mL)on various tumor cells,including SMMC-7721,HepG2,H22,CAL27,MCF7,HT29,and HCT116,were assessed.Effects of TAG on HCC proliferation and apoptosis were detected by colony formation assays and cell stainings.Caspase-3,Bcl-2,and Bax protein levels were detected by Western blotting.In vivo,a tumor xenograft model was developed using H22 cells.Totally 40 Kunming mice were randomly assigned to model,cyclophosphamide(20 mg/kg),TAG low-dose(TAG-L,0.5 mg/kg),and TAG high-dose(TAG-H,1 mg/kg)groups,with 10 mice in each group.Tumor volume,body weight,and tumor weight were recorded and compared during 14-day treatment.Immune organ index were calculated.Tissue changes were oberseved by hematoxylin and eosin staining and immunohistochemistry.Additionally,transcriptomic and metabolomic analyses,as well as quatitative real-time polymerase chain reaction(RT-qPCR),were performed to detect mRNA and metabolite expressions.Results:HPLC successfully identified the components of TAG extraction.Live cell imaging and analysis,along with cell viability assays,demonstrated that TAG inhibited the proliferation of SMMC-7721,HepG2,H22,CAL27,MCF7,HT29,and HCT116 cells.Colony formation assays,Hoechst 33258 staining,Rhodamine 123 staining,and Western blotting revealed that TAG not only inhibited HCC proliferation but also promoted apoptosis(P＜0.05).In vivo experiments showed that TAG inhibited the growth of solid tumors in HCC in mice(P＜0.05).Transcriptomic analysis and RT-qPCR indicated that the inhibition of HCC by TAG was associated with the regulation of the key gene CXCL13.Conclusion:TAG inhibits HCC both in vivo and in vitro,with its inhibitory effect linked to the regulation of the key gene CXCL13.关键词
hepatocellular carcinoma/Gelsemium elegans Benth./alkaloids/CXCL13/Chinese medicine/traditional Yao medicineKey words
hepatocellular carcinoma/Gelsemium elegans Benth./alkaloids/CXCL13/Chinese medicine/traditional Yao medicine引用本文复制引用
JIN Ming-jing,LI Yan-ping,ZHOU Huan-si,ZHAO Yu-qian,ZHAO Xiang-pei,YANG Mei,QIN Mei-jing,LU Chun-hua..Suppression of Hepatocellular Carcinoma through Apoptosis Induction by Total Alkaloids of Gelsemium elegans Benth.[J].中国结合医学杂志（英文版）,2025,31(9):792-801,10.基金项目
Supported by the Innovation Project of Guangxi Graduate Education(No.YCSW2023388),and Guangxi University of Chinese Medicine PhD Start-up Fund Project(No.2020BS034) （No.YCSW2023388）
