种子2025，Vol.44Issue(7)：1-10,48,11.DOI:10.16590/j.cnki.1001-4705.2025.07.001
基于高能碳离子辐射的野生大豆突变群体百粒重GWAS分析
Genome-wide Association Analysis of the 100-grain Weight of Wild Soybean Mutant Populations Based on High-energy Heavy Ion Radiation
摘要
Abstract
The 100-grain weight is one of the three elements that constitute the yield of soybeans(Gly-cine max).The weight per 100-grain determines the yield of soybeans and affects their commercial quality.The 100-grain weight is also a key trait for genetic improvement of soybeans.To further ex-plore the genes related to grain size and analyze the regulatory mechanism of grain size.By radiating wild soybeans(ZYD7068)with a Carbon Ion Beam(CIB),a mutant population of 200 wild soybeans with rich 100-grain weight variations was constructed.The results showed that the 100-grain weight of the population grains ranged from 4.37 g to 16.74 g,with an average of 7.13 g.The 100-grain weight of the mutant population was 4.98 times higher than that of wild soybeans.Whole-genome se-quencing was performed on 200 mutants,obtaining a total of 18.41 billion bp of sequencing data,with genomic coverage ranging from 68.10%to 70.96%.Mutations were mainly single-base mutations(SNP),among which C to T mutations account for 40%of the total number of mutations.Enrich-ment analysis of candidate SNP genes was conducted,and the mutant genes were mainly enriched in pathways such as Go:0009626(plant hypersensitivity reaction),Go:0034050(symbiont-induced host programmed cell death),Go:0012501(programmed cell death),Go:0043680(filamentous organs),and Go:0045087(innate immune response).Using SNP markers obtained from whole-genome sequen-cing and the MLM model,a genome-wide association was conducted on the 100-grain weight of the mutant population.A total of 71 associated SNPs were detected(-lgP>11).They were respectively located on chromosomes 2,3,5,6,9,10,14,16 and 18 of soybeans.Among them,9,21,9 and 23 SNPs were respectively detected on chromosomes 5,10,14 and 18.The 71 associated SNPs loci in-volved 33 genes.关键词
大豆/百粒重/GWASKey words
Glycine max/100-grain weight/genome-wide association analysis分类
农业科技引用本文复制引用
刘秀林,张必弦,赵克臻,王雪扬,张丰屹,袁荣强,张春蕾,任洪雷,苗丽丽,王家军..基于高能碳离子辐射的野生大豆突变群体百粒重GWAS分析[J].种子,2025,44(7):1-10,48,11.基金项目
黑龙江省重点研发计划(JD2023SJ28) （JD2023SJ28）
黑龙江省创新跨越工程(CX23TS04、CX23ZD04) （CX23TS04、CX23ZD04）
黑龙江省科研院所科研业务费项目(CZ-KYF2023-1-A001) （CZ-KYF2023-1-A001）
"十四五"重点研发计划项目(2022YFD1200704-2) （2022YFD1200704-2）
