中南民族大学学报（自然科学版）2025，Vol.44Issue(6)：802-809,8.DOI:10.20056/j.cnki.ZNMDZK.20250702
采用分子标记和次级代谢产物研究利川大黄的道地性
The authenticity research of Rhubarb Lichuan based on molecular markers and secondary metabolites
摘要
Abstract
Lichuan Rhubarb faces the problems of wild resource depletion,variety degradation and unclear genetic background,which seriously restricts the sustainable development and brand value of Lichuan Rhubarb industry.ISSR and RAPD molecular markers were used to evaluate the authenticity of Rhubarb Lichuan in combination with the signature secondary metabolites.Twelve ISSR and eight RAPD primers were screened,and the combination of ISSR and RAPD primers showed that five primers could be used to distinguish Rhubarb Lichuan from Rhubarb from other regions.Rhubarb in Lichuan and other regions were divided into different groups based on cluster analysis,and Rhubarb in Lichuan was divided into two categories,indicating that the genetic diversity of Rhubarb germplasm resources was correlated with the geographical distribution,and was significantly affected by climate environment.HPLC results showed that the contents of emodin,chrysophanol and emodin methyl ether were higher in Lichuan Rhubarb than in other areas.In this study,genetic diversity of Rhubarb from Lichuan and other areas was analyzed by molecular markers,and the kinship relationship between Rhubarb from Lichuan and other areas was preliminarily revealed,which provided an effective means for the identification and identification of Rhubarb from Lichuan as a landmark product,and provided theoretical basis and technical support for the research on the authenticity of Rhubarb resources in Lichuan.关键词
大黄/分子标记/遗传多样性/道地性Key words
Rheum officinale/molecular marker/genetic diversity/authenticity分类
生物科学引用本文复制引用
吴琳绯,居楚翔,陈久明,李立忠,刘虹,覃永华..采用分子标记和次级代谢产物研究利川大黄的道地性[J].中南民族大学学报（自然科学版）,2025,44(6):802-809,8.基金项目
中央高校基本科研业务费专项资金资助项目(CZY23002,CZZ25006) （CZY23002,CZZ25006）
中南民族大学校级科研重点机构平台资助项目(PTZ24021,PTZ25018) （PTZ24021,PTZ25018）
