中南民族大学学报（自然科学版）2025，Vol.44Issue(6)：817-825,9.DOI:10.20056/j.cnki.ZNMDZK.20250820
民间饮料植物红芽木叶抗急性肠胃炎作用机制研究
Cratoxylum formosum ssp.pruniflorum alleviates inflammatory response by ROS production and PI3K-Akt signaling pathway
摘要
Abstract
To investigate the mechanism of Cratoxylum formosum ssp.pruniflorum(CF)against acute gastroenteritis by using network pharmacology,molecular docking,and in vitro experimental validation.The active ingredients were collected by combining literatures and databases,and the disease targets of acute gastroenteritis were obtained.Next,the protein-protein interaction network and the"active ingredient of CF-target"network were mapped,then GO enrichment analysis and KEGG pathway enrichment analysis were performed.Additionally,molecular docking on the core targets and key components of CF were performed.In vitro experiments,the phenolic and flavonoid content of CF leaves were measured,and then antioxidant capacity of CF leaves was evaluated by DPPH,ABTS and FRAP methods.A lipopolysaccharide(LPS)-induced inflammation model of RAW264.7 cells was established to assess the anti-inflammatory activity of CF.The release of NO,TNF-α and IL-6 were detected by Griess method and ELISA method,respectively.The level of reactive oxygen species in the cells was measured also,and the expression and phosphorylation levels of Akt and PI3K were determined by West Blot.Network pharmacological analysis revealed that CF leaves could act on core targets(such as STAT3,SRC,PIK3CA,HSP90AA1,MAPK1,AKT1,et al)through key active ingredients(such as Vismione D,Vismione B,Madagascine,Parietin,Vismiaquinone A),and regulate PI3K-Akt signaling pathway,Rap1 signaling pathway,AGE-RAGE signaling pathway and other pathways to play an anti-acute gastroenteritis effect.CF demonstrated a high total phenolic and flavonoid content and a strong antioxidant potential,according to the results of in vitro research.The cellular experiment showed that CF leaves may significantly diminish the release of inflammatory factors NO,TNF-α and IL-6 induced by LPS,decrease intracellular ROS levels,and inhibit the phosphorylation of PI3K and Akt.Conclusion:CF leaves may exert therapeutic effects on acute gastroenteritis by reducing intracellular oxidative stress and inhibiting the activation of PI3K-Akt signaling pathway through multi-components and multi-targets,which provides experimental basis for the further development and utilization of of CF.关键词
红芽木/急性肠胃炎/网络药理学/氧化应激/PI3K-Akt信号通路Key words
Cratoxylum formosum ssp.pruniflorum/acute gastroenteritis/network pharmacology/oxidative stress/PI3K-Akt signaling pathway分类
医药卫生引用本文复制引用
宋雅雅,龙春林,陈玮哲,李昕阳,冯丹丹,刘立亚..民间饮料植物红芽木叶抗急性肠胃炎作用机制研究[J].中南民族大学学报（自然科学版）,2025,44(6):817-825,9.基金项目
国家自然科学基金资助项目(32370407,31870316) （32370407,31870316）
中央民族大学本科生创新训练计划项目(URTP2023110067) （URTP2023110067）
民族地区生态环境国家民委重点实验室(中央民族大学)自主课题(KLEEMA202202) （中央民族大学）
