川北医学院学报2025，Vol.40Issue(8)：968-972,5.DOI:10.3969/j.issn.1005-3697.2025.08.003
举一例介绍光学分合法用于估计器官内细胞总数
Use of the optical fractionator(unbiased stereological method)exempli-fied by total number estimation of cells in an organ
何琦 1唐勇 2杨正伟3
作者信息
- 1. 川北医学院基础医学研究中心人体解剖学教研室,四川 南充 637000
- 2. 重庆医科大学组织学与胚胎学教研室,重庆渝中 400016
- 3. 川北医学院形态定量研究室,四川 南充 637000
- 折叠
摘要
Abstract
For promotion of the optical fractionator,an unbiased stereological method of the optical disector combined with the fractionator that is commonly used to estimate the total number of cells in an organ(tissue),the present article exemplified its use in a total number estimation of neurons.Serial frozen sections of the dentate gyrus(DG region)of the hippocampal formation on one side were obtained from an adult mouse,1/5 of the sections were sampled in a systematic random manner,with 14 sections being sampled.The sections were stained using an immunohistochemical method and counterstained with cresyl violet to show DCX-positive neurons.The whole DG regions were observed and the somas(cell bodies)of DCX-positive neurons were counted within 15 μm(thickness)of the section tissue with the optical disector,meanwhile the actual thickness of the section tissue was measured with a microcator.As a re-sult,291 neurons(somas)were counted and the average of measured section thicknesses was 24.22 μm.Therefore,according to the op-tical fractionator,the total number of DCX-positive neurons in the hippocampal DG region was:291×(5/1)×(24.22/15)=2,439.With this example,we discussed in more details about the theoretical foundation and practice-related issues of the optical fractionator.关键词
光学分合法/海马/神经元/数量/体视学Key words
Optical fractionator/Hippocampus/Neurons/Number/Stereology分类
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何琦,唐勇,杨正伟..举一例介绍光学分合法用于估计器官内细胞总数[J].川北医学院学报,2025,40(8):968-972,5.
