临床与病理杂志2025，Vol.45Issue(5)：527-538,12.DOI:10.11817/j.issn.2095-6959.2025.250024
DAXX在脊索瘤中的表达及其与脊索瘤生物学行为的相关性
Expression of DAXX in chordoma and its correlation with the biological behavior
摘要
Abstract
Objective:Chordoma is a rare malignant tumor characterized by a high recurrence rate and poor prognosis.This study aims to investigate the expression of death domain-associated protein(DAXX)in chordoma and its correlation with tumor biological behavior and clinical significance. Methods:A total of 156 chordoma specimens surgically resected between January 2016 and January 2022 were collected,among which 144 cases had complete follow-up data and 12 were lost to follow-up.In addition,18 adjacent non-tumor tissue samples were included.Hematoxylin-eosin and immunohistochemical staining were performed to analyze DAXX expression in 144 chordoma tissues and 18 adjacent tissues.The relationship between DAXX expression and clinicopathological parameters was examined,and the prognostic outcomes of DAXX-positive patients were evaluated.In chordoma cell lines(U-CH1,MUG-Chor1),DAXX knockdown was achieved,followed by western blotting and quantitative polymerase chain reaction(qPCR)to assess DAXX expression.The effects on cell proliferation were evaluated by MTT and colony formation assays,while migration and invasion were assessed by wound healing and Transwell assays. Results:High DAXX expression was observed in 91 cases(63.2%)of chordoma and low expression in 53 cases(36.8%);all 18 adjacent tissues showed low expression.DAXX expression was correlated with primary/relapsed tumor status and tumor size(both P<0.05),but not with other clinical features.Log-rank test analysis revealed that patients with high DAXX expression had significantly lower progression-free survival(PFS)and overall survival(OS)compared with those with low DAXX expression(both P<0.05).Cox regression analysis identified DAXX expression level,tumor primary/relapse status,surgical method,and tumor size as independent risk factors for postoperative progression(all P<0.05).In vitro,DAXX knockdown significantly suppressed chordoma cell proliferation,invasion,and migration(all P<0.001). Conclusion:High DAXX expression in chordoma is closely associated with poor prognosis,while DAXX knockdown inhibits tumor aggressiveness.DAXX may serve as a predictive marker for tumor progression and prognosis in chordoma patients.关键词
死亡结构域相关蛋白/脊索瘤/预后/生物学行为/免疫组织化学染色Key words
death domain-associated protein/chordoma/prognosis/biological behavior/immunohistochemical staining引用本文复制引用
姚正奇,陈素,矫健,杨明磊,钟南哲,肖建如..DAXX在脊索瘤中的表达及其与脊索瘤生物学行为的相关性[J].临床与病理杂志,2025,45(5):527-538,12.基金项目
国家自然科学基金(81902731 （）
82173028).This work was supported by the National Natural Science Foundation of China(81902731 （）
82173028). （）
