海南医科大学学报2025，Vol.31Issue(16)：1211-1219,9.DOI:10.13210/j.cnki.jhmu.20241015.001
俞募配穴对少弱精子症大鼠睾丸TGF-β/smads信号通路的影响
Effects of Shumu acupoints on TGF-β/smads signaling pathway in testis of oligoasthenospermia rats
摘要
Abstract
Objective:To observe the effects of Shumu Naoshen combined with acupoint electroacupuncture on oligoastheno-spermia model rats,and to explore the effects of Shumu Naoshen combined with acupoint electroacupuncture on the reproductive function of oligoasthenospermia rats as well as the influence of TGF-β/smads signaling pathway on testis.Methods:A total of 40 male SD rats were randomly divided into the blank group,the model group,the electroacupuncture group,and the L-carnitine group,with 10 rats in each group.Except for the blank group,the oligoasthenospermia rat model was prepared by adenine instilla-tion for 28 d.After a successful modeling,the electroacupuncture group(3 lines of frontal injection,Shenshu,Jingmen,and Taixi injection,30 min∙time-1∙d-1,for 28 d)and the L-carnitine group(10 mL/kg L-carnitine oral infusion,for 28 d)were respectively administered.After the treatment,the sperm density and motility rate of epididymis were tested.The morphological changes of testis were observed by H&E staining.Serum sex hormones testosterone,follicle-stimulating hormone,luteinizing hormone,and estradiol were detected by ELISA.The expression levels of TGF-β,P-smad2,and P-smad3 in testicular tissues were detected by Western blot.Results:Compared to the blank group,spermatogenic cells in testis tissue of model group were degenerated,the number was decreased and the arrangement was disordered.Sperm density,sperm motility rate,and serum testosterone level were significantly decreased(P<0.05),luteinizing hormone level was significantly increased(P<0.05).Compared to the model group,the pathological damage of testicle in L-carnitine group and electroacupuncture group was significantly reduced;sperm den-sity,motility rate,and serum testosterone level were significantly increased(P<0.05),and luteinizing hormone level was signifi-cantly decreased(P<0.05).Conclusion:Shumu Naoshen combined with acupoint electroacupuncture can increase sperm density and viability,regulate sex hormone production,and improve spermatogenesis environment of rats with oligoasthenospermia.The mechanism may be related to the regulation of serum sex hormone levels and the influence of TGF-β/smads signaling pathway on testis spermatogenesis.关键词
少弱精子症/俞募配穴/性激素/TGF-β/smads信号通路Key words
Oligoasthenospermia/Shumu acupoints/Sex hormone/TGF-β/smads signaling pathway分类
医药卫生引用本文复制引用
王菲,郭斌,孟纯雪,孙文静,杨楠,赵海龙,张生富,张千,唐旭红,马惠昇..俞募配穴对少弱精子症大鼠睾丸TGF-β/smads信号通路的影响[J].海南医科大学学报,2025,31(16):1211-1219,9.基金项目
This study was supported by the 2021 Autonomous Region Key Research and Development Plan(2021BEG020412)and School Level Project of Ningxia Medical University(XZ2021004) 2021年自治区重点研发计划资助项目(2021BEG02041) （2021BEG020412）
宁夏医科大学校级课题资助项目(XZ2021004) （XZ2021004）
