吉林农业大学学报2025，Vol.47Issue(4)：645-653,9.DOI:10.13327/j.jjlau.2025.0106
激光补光对黄瓜叶片叶绿素荧光性能、细胞结构及转录组的影响
Effects of Laser Supplemental Lighting on Chlorophyll Fluorescence Properties,Cellular Structure,and Transcriptome of Cucumber Leaves
摘要
Abstract
To investigate the effects of laser supplemental lighting on chlorophyll fluorescence prop-erties,cell structure and transcriptome of cucumber leaves,and to provide a theoretical basis for the application of laser supplemental lighting in cucumber cultivation,"Chuancui 13"cucumber was used as the experimental material,and laser supplemental lighting and control groups were estab-lished.By measuring leaf area,chlorophyll content,chlorophyll fluorescence parameters,cell struc-ture and transcriptome,the effects of laser supplemental lighting on cucumber leaf morphology,physiology,and molecular level were in-depth analyzed.The results showed that laser supplemental lighting significantly enhanced cucumber leaf area,total chlorophyll content,and specific leaf weight,with increases of 11.6%,13.6%,and 15.4%,respectively.However,it did not significantly af-fect PSⅡ energy distribution and photosynthetic performance index.Observations of leaf cell struc-ture showed that laser supplemental lighting increased the thickness of palisade tissue and the inter-cellular spaces of spongy tissue and promoted the increase in the size and number of starch grains in chloroplasts.Transcriptome analysis revealed that differentially expressed genes were mainly en-riched in cell wall synthesis and carbohydrate metabolism processes,and KEGG metabolic pathways were mainly enriched in brassinosteroid biosynthesis,nitrogen metabolism,zeatin biosynthesis,gib-berellin synthesis,and starch and sucrose metabolism pathways.The expression levels of genes re-lated to zeatin,gibberellin,and starch synthesis were significantly up-regulated.In conclusion,laser supplemental lighting promoted cucumber leaf growth by regulating plant hormone metabolism and carbohydrate metabolism,enhanced photosynthesis,and ultimately increased the content of assimi-lates in cucumber leaves.关键词
激光/黄瓜/光合性能/细胞结构/转录组Key words
laser/cucumber/photosynthesis/cell structure/transcriptome分类
农业科技引用本文复制引用
张泽锦,梁颖,王剑锋,唐丽,文熙宸..激光补光对黄瓜叶片叶绿素荧光性能、细胞结构及转录组的影响[J].吉林农业大学学报,2025,47(4):645-653,9.基金项目
四川省农业农村厅项目(SCCXTD-2024-5),四川省科技厅蔬菜育种攻关项目(2021YFYZ0022) （SCCXTD-2024-5）
