实用心电与临床诊疗2025，Vol.34Issue(4)：494-500,7.DOI:10.13308/j.issn.2097-5716.2025.04.004
lncZFAS1调节细胞自噬在心肌细胞纤维化中的作用
Role of lncZFAS1 in regulating cellular autophagy in cardiomyocyte fibrosis
摘要
Abstract
Objective To investigate the impact of long non-coding RNA ZFAS1(lncZFAS1)on the initiation and progression of cardiomyocyte fibrosis.Methods HL-1 mouse cardiac myocytes were induced by transforming growth factor-β(TGF-β)to establish a cardiomyocyte fibrosis model.LncZFAS1 overexpression vectors or small interfering RNA(siRNA)were transfected into the model.Protein levels of α-smooth muscle actin(α-SMA)and collagen Ⅰ were detected by western blotting;lncZFAS1 overexpression and interference fragments were transfected into the cardiomyocyte fibrotic model.The transcriptional level of lncZFAS1 was measured via real-time quantitative PCR.Cell proliferation viability was assessed using the CCK-8 assay,apoptosis rate was analyzed by flow cytometry,and LC3 autophagy puncta formation was examined using cellular immunofluorescence.Results Compared with the control group,the TGF-β induction group showed significantly increased protein expression of α-SMA,collagen Ⅰ and LC3 B,significantly decreased p62 protein expression,elevated lncZFAS1 expression,enhanced cell viability and autophagy puncta formation,and reduced apoptosis rate(all P＜0.05).After interfering with lncZFAS1 in the model,it showed a decrease in the expression level of lncZFAS1,a decrease in cell viability and the number of autophagy puncta,an increase of apoptosis rate and expression of p62 protein,and a decrease in the expression of LC3 B protein,all with statistically significant differences(all P＜0.05).However,after overexpression of lncZFAS1,the opposite results were presented.Conclusion LncZFAS1 promotes the initiation and progression of cardiomyocyte fibrosis by regulating cellular autophagy.Targeted inhibition of lncZFAS1 may provide a novel therapeutic strategy for delaying the progression of cardiovascular diseases.关键词
心肌细胞纤维化/长链非编码RNA ZFAS1/自噬/α-平滑肌肌动蛋白/Ⅰ型胶原蛋白Key words
cardiomyocyte fibrosis/long non-coding RNA ZFAS1/autophagy/α-smooth muscle actin/collagen Ⅰ分类
医药卫生引用本文复制引用
林小花,欧阳煜,程效,肖婧雯,江宇,魏沁菲,张彦..lncZFAS1调节细胞自噬在心肌细胞纤维化中的作用[J].实用心电与临床诊疗,2025,34(4):494-500,7.基金项目
福州市科技计划项目(2022-S-024) （2022-S-024）
福州市"十四五"临床专科培强培优培育建设项目(20220101) （20220101）
