农业生物技术学报2025，Vol.33Issue(9)：1873-1882,10.DOI:10.3969/j.issn.1674-7968.2025.09.001
水稻Waxy基因第四内含子3'剪接位点邻近碱基突变影响水稻抗性淀粉含量
The Impact on Resistant-starch Content for Base Mutations Near the 3'Splice Site of the Fourth Intron of the Waxy Gene in Rice(Oryza sativa)
摘要
Abstract
In recent years,with the rising incidence of type Ⅱ diabetes,resistant starch(RS)has gained significant attention as a low-glycemic index food component.Resistant starch offers important physiological benefits,such as reducing postprandial blood glucose levels in diabetic patients and decreasing the risk of intestinal dysfunction and colon cancer.The content of resistant starch in rice(Oryza sativa)is positively correlated with amylose content,and the synthesis of amylose is controlled by the Waxy(Wx)gene.In this study,CRISPR/Cas9 gene editing technology was employed to edit the bases near the 3'splice site AG of the fourth intron of the Wx gene in rice,resulting in 4 different types of mutant lines.By analyzing the expression levels of the Wx gene,the activity of granule-bound starch synthase(GBSS),and the contents of amylose and resistant starch in these mutant lines,it was found that mutations near the 3'splice site AG significantly affected the expression of the Wx gene,thereby regulating the contents of amylose and resistant starch in rice.Correlation analysis revealed a significant positive correlation between Wx gene expression and resistant starch content.This study identified intron mutation genotypes that can effectively regulate the expression level of the Wx gene(either increasing or decreasing it),providing new insights for future improvements in the resistant starch content of rice.关键词
水稻/抗性淀粉/Waxy/基因编辑Key words
Rice/Resistant starch/Waxy/Gene editing分类
农业科技引用本文复制引用
罗曦,张建福,樊佳星,魏毅东,魏林艳,朱永生,何炜,吴方喜,蔡秋华,谢华安..水稻Waxy基因第四内含子3'剪接位点邻近碱基突变影响水稻抗性淀粉含量[J].农业生物技术学报,2025,33(9):1873-1882,10.基金项目
福建省属公益类科研院所专项(2022R1023001) （2022R1023001）
中央引导地方科技发展专项(2022L3018) （2022L3018）
福建省人民政府-中国农业科学院协同创新工程"5511"项目(XTCXGC2021019) （XTCXGC2021019）
福建省财政专项-福建省农业科学院科技创新团队(CXTD2021005-3) （CXTD2021005-3）
福建省科技重大专项(2024NZ029027) （2024NZ029027）
