四川农业大学学报2025，Vol.43Issue(4)：893-905,13.DOI:10.16036/j.issn.1000-2650.202412585
施氮对滇中高山栎林土壤酶活性和微生物生物量碳氮的影响
Dynamic Effects of Nitrogen Deposition on Soil Enzyme Activity and Microbial Characteristics of Quercus aquifolioides Forest in Central Yunnan
摘要
Abstract
[Objective]This study investigated the dynamic changes in polyphenol oxidase and catalase activitives,microbial biomass carbon(MBC)and nitrogen(MBN),soil nutrients,and soil organic car-bon(SOC)under nitrogen addition,with the aim of providing insights into the impact of nitrogen deposi-tion on the soil microecological environment of forest ecosystems.[Method]A Quercus aquifolioides for-est in the central Yunnan Plateau was selected as the study site.Four nitrogen addition treatments were established:control[CK,0 g/(m2·a)],low nitrogen[LN,10 g/(m2·a)],medium nitrogen[MN,20 g/(m2·a)],and high nitrogen[HN,25 g/(m2·a)].Soil samples were collected from three depths(0-10,10-20,20-30 cm)to assess changes in soil nutrients,enzyme activity,soil microbial quantity,MBC and MBN.[Result]Nitrogen addition duration did not result in significant temporal trends for most indicators.However,nitrogen concentration significantly affected polyphenol oxidase,soil pH,to-tal nitrogen(TN),ammonium nitrogen(NH4+-N),nitrate nitrogen(NO3--N),MBC and MBN(P<0.001),and had a significant impact on SOC(P<0.05).Soil depth very significantly influenced polyphe-nol oxidase,catalase,soil pH,TN,available nitrogen(AN),NH4+-N,MBC,and MBN(P<0.001);①After 2 years of nitrogen addition,SOC decreased by 10.93%-25.33%with increasing nitrogen concen-tration,while soil total phosphorus(TP)decreased by 13.09%～15.74%under HN.In contrast,TN,AN,NH4+-N and NO3--N increase by 9.70%-27.72%,4.11%-14.72%,16.36%-51.50%,17.57%-59.52%,and 22.07%-93.55%,respectively;② With larger decreases under higher nitrogen levels,Polyphenol oxidase and catalase activities declined by 3.76%-24.86%and 6.14%-29.05%,respectively;③ Com-pared to CK,the dec rease in microbial numbers in each soil layer was 16.67%-64.75%as nitrogen ap-plication concentration increased;MBC and MBN decreased by 3.75%-40.53%and 2.14%-94.99%,repsectively;④ Across all treatments,soil nutrient content,enzyme activities,MBC,and MBN de-clined with soil depth.[Conclusion]Nitrogen addition was significantly negatively correlated with SOC,but markedly promoted TN,AN,NH4+-N,and NO3--N,leading to an soil nutrient imbalances.These changes indirectly reduced the activities of polyphenol oxidase and catalase and altered the soil microbial environment in Quercus semacarpifolia forest.关键词
施氮/多酚氧化酶活性/过氧化氢酶活性/微生物生物量碳氮/滇中地区Key words
nitrogen deposition/polyphenoloxidase activity/catalase activity/microbial biomass carbon and nitrogen/central Yunnan分类
农业科技引用本文复制引用
梁子琪,王克勤,宋娅丽,陈蕾西,梁阳森,周敬..施氮对滇中高山栎林土壤酶活性和微生物生物量碳氮的影响[J].四川农业大学学报,2025,43(4):893-905,13.基金项目
云南省科技厅面上项目(202401AT070262) （202401AT070262）
云南省大学生创新创业训练计划项目(202210677011) （202210677011）
