山西医科大学学报2025，Vol.56Issue(7)：751-757,7.DOI:10.13753/j.issn.1007-6611.2025.07.004
RAB42对卵巢癌细胞生物学功能及上皮间充质转化的影响
Effect of RAB42 expression on biological function and epithelial mesenchymal transition of ovarian cancer cells
摘要
Abstract
Objective To investigate the expression and prognostic value of Ras-related protein RAB42 in ovarian cancer,and ex-plore its effect on the proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)of ovarian cancer cells.Methods The expression of RAB42 in ovarian cancer tissues and its effect on the survival of ovarian cancer patients were analyzed based on bioinformatics database.Fifty-five cases of ovarian cancer tissues and 23 cases of normal ovarian tissues were collected from Shanxi Bethune Hospital to detect the expression level of RAB42 using RT-qPCR,and analyze its relationships with the clinicopatho-logical characteristics and the prognosis.A SKOV-3 ovarian cancer cell model with downregulated RAB42 expression was constructed by transfection with lentiviral vector,CCK-8,cell scratch and Transwell assays were used to detect the proliferation,migration and in-vasion of ovarian cancer cells,and Western blot was used to detect the expressions of EMT-related proteins.Results Compared to normal ovarian tissues,the expression of RAB42 in ovarian cancer tissues was significantly upregulated(P＜0.05),and the ovarian can-cer patients with high RAB42 expression had a shorter overall survival(P＜0.05).After the expression of RAB42 gene was downregu-lated,the proliferation,migration,and invasion abilities of ovarian cancer cells were significantly reduced(P＜0.05),and the expres-sion of EMT-related protein E-cadherin was significantly increased(P＜0.05),while the expressions of N-cadherin,Ki-67,and Vimen-tin were significantly decreased(P＜0.05).Conclusion RAB42 gene is highly expressed in ovarian cancer and is associated with poor prognosis of patients.Knockdown of RAB42 gene can inhibit the proliferation,migration and invasion of ovarian cancer cells and the EMT process,and RAB42 may become a potential therapeutic target for ovarian cancer.关键词
卵巢癌/RAB42/预后/增殖/迁移/侵袭/上皮-间充质转化Key words
ovarian cancer/RAB42/prognosis/proliferation/migration/invasion/epithelial-mesenchymal transition(EMT)分类
医药卫生引用本文复制引用
钟雨轩,成思蓉,杨银霞,王赞宏..RAB42对卵巢癌细胞生物学功能及上皮间充质转化的影响[J].山西医科大学学报,2025,56(7):751-757,7.基金项目
山西省基础研究计划项目(202103021224352) （202103021224352）
中央引导地方科技发展基金项目(YDZJSX2022B012) （YDZJSX2022B012）
